Deregulation of a distinct set of microRNAs is associated with transformation of gastritis into MALT lymphoma
Deregulation of a distinct set of microRNAs is associated with transformation of gastritis into MALT lymphoma
复制标题
DOI：
10.1007/s00428-012-1215-1
复制
发表时间：
2012-04-01
期刊：
影响因子：
3.5
通讯作者：
Bernd, Heinz-Wolfram
中科院分区：
文献类型：
--
作者：
Thorns, Christoph;Kuba, Johannes;Bernd, Heinz-Wolfram
从慢性幽门螺杆菌胃炎转变为胃结外边缘区淋巴瘤（MALT淋巴瘤）的机制尚不清楚。本研究旨在鉴定可能参与肿瘤转化过程的microrna。根据Wotherspoon标准，我们通过RT-PCR在68个胃活检样本中产生了microRNA特征，这些样本代表了正常粘膜、胃炎、可疑淋巴浸润和明显的MALT淋巴瘤。分析显示，从正常粘膜到胃炎和MALT淋巴瘤，共有41个microrna依次显著上调（n = 33）或下调（n = 8）。虽然其中一些仅反映淋巴细胞的存在（如miR-566和miR-212）或幽门螺杆菌感染（如miR-155和let7f），但一组不同的五种microrna （miR-150, miR-550, miR-124a， miR-518b和miR-539）被证明在胃炎中与MALT淋巴瘤中表达差异。因此，这种差异表达可能表明这些microrna在恶性转化过程中的核心作用。
The mechanisms underlying the transformation from chronic Helicobacter pylori gastritis to gastric extranodal marginal zone lymphoma (MALT lymphoma) are poorly understood. This study aims to identify microRNAs that might be involved in the process of neoplastic transformation. We generated microRNA signatures by RT-PCR in 68 gastric biopsy samples representing normal mucosa, gastritis, suspicious lymphoid infiltrates, and overt MALT lymphoma according to Wotherspoon criteria. Analyses revealed a total of 41 microRNAs that were significantly upregulated (n = 33) or downregulated (n = 8) in succession from normal mucosa to gastritis and to MALT lymphoma. While some of these merely reflect the presence of lymphocytes (e.g. miR-566 and miR-212) or H. pylori infection (e.g. miR-155 and let7f), a distinct set of five microRNAs (miR-150, miR-550, miR-124a, miR-518b and miR-539) was shown to be differentially expressed in gastritis as opposed to MALT lymphoma. This differential expression might therefore indicate a central role of these microRNAs in the process of malignant transformation.