Molecular evidence for the rapid propagation of mouse t haplotypes from a single, recent, ancestral chromosome.
Molecular evidence for the rapid propagation of mouse t haplotypes from a single, recent, ancestral chromosome.
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DOI：
10.1093/oxfordjournals.molbev.a040457
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发表时间：
1987-09
影响因子：
10.7
通讯作者：
L. Silver;M. Hammer;H. Fox;J. Garrels;M. Bucan;B. Herrmann;A. Frischauf;H. Lehrach;H. Winking;F. Figueroa
中科院分区：
文献类型：
--
作者：
L. Silver;M. Hammer;H. Fox;J. Garrels;M. Bucan;B. Herrmann;A. Frischauf;H. Lehrach;H. Winking;F. Figueroa
小鼠单倍型是17号染色体的变异形式，在世界范围内两种共生小鼠种群中存在频率很高。为了确定t单倍型之间的相互关系以及它们所在的物种，我们利用10个独立的分子探针对35个具有代表性的t单倍型进行了分析，其中包括5个DNA克隆和5个二维凝胶电泳鉴定的多肽点。所有测试的单倍型都发现具有限制性片段和多肽点，而这些片段和多肽点在携带17号染色体野生型的小鼠中是不存在的。这一观察提供了第一个直接证据，证明所有已知的t单倍型都是单一祖先染色体的后代。t单倍型之间没有变异可能意味着这条祖先染色体存在的时间相对较晚，在这种情况下，有必要假设t单倍型在物种系之间的渗入，以解释它们在家鼠和家鼠中的存在。另一种可能是，祖先的染色体在家蝇和肌蝇分裂之前就存在了，而我们的探针碰巧没有检测到t单倍型中存在的多态性。我们分析的进一步结果是在以色列小鼠野生种群中部分t单倍型的特征。
Mouse t haplotypes are variant forms of chromosome 17 that exist at high frequencies in worldwide populations of two species of commensal mice. To determine both the relationship of t haplotypes to each other and the species within which they exist, 35 representative t haplotypes were analyzed by means of 10 independent molecular probes, including five DNA clones and five polypeptide spots identified by means of two-dimensional gel electrophoresis. All of the tested haplotypes were found to share restriction fragments and polypeptide spots that are absent in mice carrying wild-type forms of chromosome 17. This observation provides the first direct evidence that all of the known t haplotypes are descendents of a single ancestral chromosome. The absence of variation among t haplotypes could mean that this ancestral chromosome existed relatively recently, in which case it would be necessary to postulate introgressions of t haplotypes across species lines to explain their presence in both Mus domesticus and M. musculus. Alternatively, it is possible that the ancestral chromosome existed prior to the split between M. domesticus and M. musculus and that, by chance, our probes fail to detect polymorphisms that exist among the t haplotypes. A further result of our analysis is the characterization of a partial t haplotype in a wild population of Israeli mice.