Highly enhanced fucosylation of serum glycoproteins in patients with hepatocellular carcinoma
Highly enhanced fucosylation of serum glycoproteins in patients with hepatocellular carcinoma
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DOI：
10.1046/j.1440-1746.1999.01882.x
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发表时间：
1999-05-01
影响因子：
4.1
通讯作者：
Asakura, H
中科院分区：
文献类型：
--
作者：
Naitoh, A;Aoyagi, Y;Asakura, H
背景：我们最近报道，甲胎蛋白 (AFP)、α1-抗胰蛋白酶 (AAT) 和转铁蛋白 (TF) 的菜豆凝集素反应性物质的测量可用于肝细胞癌 (HCC) 的诊断，并且这种反应性的分子基础是双触角糖链最内部的 N-乙酰氨基葡萄糖残基的岩藻糖基化。然而，HCC和肝硬化患者中AFP、AAT和TF岩藻糖基化的确切关系尚不完全清楚。本研究的目的是阐明这三种糖蛋白在 HCC 中的岩藻糖基化之间的关系。方法：1987 年至 1997 年间转诊到我们大学医院的 334 名 HCC 患者。在 233 名 (69.8%) HCC 患者中观察到血清 AFP 升高 (> 20 ng/mL)。从这 233 名产生 AFP 的 HCC 患者中，随机选择了 60 份血清样本用于本研究。作为参考，使用了 60 名肝硬化患者的样本，其中 30 名患者的 AFP 升高。通过交叉免疫亲和电泳（CIAE）测定小扁豆凝集素（LCA）反应种类。通过高效液相色谱 (HPLC) 测定纯化的 AAT 和 TF 样品中岩藻糖基化双触角链的含量，以每种寡糖的吡啶氨基衍生物的形式测定。结果：AAT 和 TF 中 CIAE 的 LCA 反应物种与 HPLC 的吡啶氨基岩藻糖基化双触角糖链之间存在高度显着的相关性。 HCC 中的菜豆凝集素反应种类 AFP、AAT 和 TF 显着高于肝硬化。在 HCC 和肝硬化中，AAT 和 TF 之间观察到岩藻糖基化聚糖之间存在高度统计学上显着的正相关性，但 AFP 和 AAT 之间或 AFP 和 TF 之间则没有观察到。因此，目前的结果表明，与肝硬化相比，在 HCC 中发现血清糖蛋白的岩藻糖基化高度增强，并且岩藻糖基化 AFP 与 AAT 或 TF 的测量组合可用于 HCC 的诊断。 (C) 1999 Blackwell Science Asia Pty Ltd。
Background: We recently reported that the measurement of Lens culinaris agglutinin-reactive species of a-fetoprotein (AFP), alpha-1-antitrypsin (AAT) and transferrin (TF) is useful for the diagnosis of hepatocellular carcinoma (HCC) and that the molecular basis for this reactivity is fucosylation at the innermost N-acetylglucosamine residue of a biantennary sugar chain. However, the precise relationship of the fucosylation of AFP, AAT and TF in patients with HCC and liver cirrhosis is not fully understood. The aim of this study is to delineate the relationship of the fucosylation between these three glycoproteins in HCC.Methods: Three hundred and thirty-four patients with HCC were referred to our university hospital from 1987 to 1997. An increase in serum AFP (> 20 ng/mL) was observed in 233 (69.8%) patients with HCC. From these 233 patients with AFP-producing HCC, 60 serum samples were randomly selected and used in the present study. As a reference, samples from 60 patients with liver cirrhosis, in which 30 had increased AFP, were used. Lens culinaris agglutinin (LCA)-reactive species were determined by crossed immunoaffinoelectrophoresis (CIAE). The contents of the fucosylated biantennary chain of purified AAT and TF samples were determined as pyridylamino derivatives of each oligosaccharide with high-performance liquid chromatography (HPLC).Results: There was a highly significant correlation between LCA-reactive species by CIAE and pyridylamino-fucosylated biantennary sugar chain by HPLC in both AAT and TF. Lens culinaris agglutinin-reactive species of AFP, AAT and TF in HCC were: significantly higher than those in liver cirrhosis. A highly statistically significant positive correlation of fucosylated glycans was observed between AAT and TF in both HCC and liver cirrhosis, but not between AFP and AAT or between AFP and TF. Accordingly, the present results indicate that highly enhanced fucosylation of serum glycoproteins was found in HCC compared with liver cirrhosis and that the combination of measurements of fucosylated AFP with AAT or TF were useful for the diagnosis of HCC. (C) 1999 Blackwell Science Asia Pty Ltd.