Testing BR photocycle kinetics.
Testing BR photocycle kinetics.
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测试 BR 光循环动力学。
DOI：
10.1016/s0006-3495(95)80321-8
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Lanyi,JK
中科院分区：
文献类型：
--
作者：
Nagle,JF;Zimanyi,L;Lanyi,JK
从之前的细菌视紫红质D96N突变体的光循环数据中获得了改进的可见光K吸收光谱。在较低温度下的数据与5℃至25℃光谱中的可忽略的温度依赖性是一致的。详细分析表明，除了第一个中间体K和最后一个中间体M之外，还存在两个中间体，假设两个中间体具有相同的光谱，并使用之前得到的L光谱。包含四个中间产物的最佳动力学模型与中间产物的时间过程相当不寻常，在死胡同上有两个L。然而，先前提出的一个更传统的包含五个中间体的模型，包括两个具有相同光谱的L和两个具有相同光谱的M，也几乎可以拟合中间体的时间过程。还提出了一种新的标准，可以根据数据来测试单个提议的频谱。
An improved K absorption spectrum in the visible is obtained from previous photocycle data for the D96N mutant of bacteriorhodopsin, and the previously obtained M absorption spectrum in the visible and the fraction cycling are confirmed at 25 degrees C. Data at lower temperatures are consistent with negligible temperature dependence in the spectra from 5 degrees C to 25 degrees C. Detailed analysis strongly indicates that there are two intermediates in addition to the first intermediate K and the last intermediate M. Assuming two of the intermediates have the same spectrum and using the L spectrum obtained previously, the best kinetic model with four intermediates that fits the time course of the intermediates is rather unusual, with two L's on a cul-de-sac. However, a previously proposed, more conventional model with five intermediates, including two L's with the same spectra and two M's with the same spectra, also fits the time course of the intermediates nearly as well. A new criterion that tests an individual proposed spectrum against data is also proposed.