Distribution comparison and risk assessment of free-floating and particle-attached bacterial pathogens in urban recreational water: Implications for water quality management
Distribution comparison and risk assessment of free-floating and particle-attached bacterial pathogens in urban recreational water: Implications for water quality management
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DOI：
10.1016/j.scitotenv.2017.09.008
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发表时间：
2018-02-01
影响因子：
9.8
通讯作者：
Ye, Quanhui
中科院分区：
文献类型：
--
作者：
Fang, Tingting;Cui, Qijia;Ye, Quanhui
娱乐用水中接触病原体的风险是全世界关注的问题。此外，这些水域中作为病原体理想庇护所的悬浮颗粒也需要关注。然而，病原体-颗粒附着造成的风险在很大程度上是未知的。在此基础上，采集了北京市三个休闲湖泊的水样，并将其分为游离态(FL，0.22-5微米)和颗粒态(PA，N5微米)两组分。用下一代测序技术(NGS)检测病原菌属的多样性，用定量聚合酶链式反应(QPCR)检测大肠埃希菌(UidA)、肠沙门氏菌(InvA)、气单胞菌(Aeromonas spp.)的基因。(AERA)、禽分枝杆菌(16S)和铜绿假单胞菌(OAA)。NGS结果表明，PA组分和FL组分具有稳定的病原菌属组成差异。一些属，如气单胞菌和分枝杆菌，在PA组分中表现出较高的丰度。定量聚合酶链式反应显示，大多数基因在颗粒内的浓度高于FL组分。一些基因水平与颗粒物浓度和湖泊营养水平相关。进一步对选定菌株(肠链球菌和禽链霉菌)的定量微生物风险评估(QMRA)表明，在营养物质和颗粒物较多的湖泊进行二次接触活动时，健康风险较高。结果表明，城市游憩水中悬浮颗粒物(主要由藻类组成)可能影响病原菌的分布，可作为病原菌污染的蓄水池，具有重要的管理意义。(C)2017爱思唯尔B.V.保留所有权利。
The risk of pathogen exposure in recreational water is a concern worldwide. Moreover, suspended particles, as ideal shelters for pathogens, in these waters also need attention. However, the risk caused by the pathogen-particle attachment is largely unknown. Accordingly, water samples in three recreational lakes in Beijing were collected and separated into free-floating (FL, 0.22-5 mu m) and particle-attached (PA, N5 mu m) fractions. Next-generation sequencing (NGS) was employed to determine the diversity of genera containing pathogens, and quantitative PCR (qPCR) was used to assess the presence of genes from Escherichia coli (uidA), Salmonella enterica (invA), Aeromonas spp. (aerA), Mycobacterium avium (16S) and Pseudomonas aeruginosa (oaa). The NGS results showed stable pathogen genera composition distinctions between the PA and FL fractions. Some genera, such as Aeromonas and Mycobacterium, exhibited higher abundances in the PA fractions. qPCR revealed that most of the gene concentrations were higher within particles than were FL fractions. Some gene levels showed correlations with the particle concentrations and lake nutrient levels. Further quantitative microbial risk assessment (QMRA) of selected strains (S. enterica and M. avium) indicated a higher health risk during secondary contact activities in lakes with more nutrients and particles. We concluded that suspended particles (mainly composed of algae) in urban recreational water might influence the pathogen distribution and could serve as reservoirs for pathogen contamination, with important management implications. (C) 2017 Elsevier B.V. All rights reserved.