AMINO-ACID ANALYSIS USING PHENYLISOTHIOCYANATE PREDERIVATIZATION - ELIMINATION OF THE DRYING STEPS
AMINO-ACID ANALYSIS USING PHENYLISOTHIOCYANATE PREDERIVATIZATION - ELIMINATION OF THE DRYING STEPS
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DOI：
10.1016/0165-022x(88)90012-7
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发表时间：
1988-03-01
影响因子：
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通讯作者：
FINLAYSON, JR
中科院分区：
文献类型：
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作者：
INGLIS, AS;BARTONE, NA;FINLAYSON, JR
本文报道了用苯基硫代氨基胺分析氨基酸的方法进行氨基酸分析的实验结果。研究发现，在盐的存在下，某些氨基酸的产率随真空干燥步骤的变化而变化。描述了一种改进的程序，其包括在干燥之前向水解物中加入标准的盐；再干燥步骤被省略，衍生化后的干燥被简单地向反应混合物中加入庚烷所取代。
Results of experiments on the procedure for amino acid analysis via analysis of the phenylthiocarbamyl amino acids are reported. It was found that yields of some amino acids varied in the presence of salt and with changes in the vacuum drying steps. An improved procedure is described which includes a standard addition of salt to the hydrolysate before drying it; the redrying step is omitted and the post derivatizatin drying is replaced by a simple addition of heptane to the reaction mixture.