Sex-Dependent Effects of Chronic Microdrive Implantation on Acquisition of Trace Eyeblink Conditioning.
Sex-Dependent Effects of Chronic Microdrive Implantation on Acquisition of Trace Eyeblink Conditioning.
复制标题
慢性微驱动器植入对微量眨眼条件获得的性别依赖性影响。
DOI：
10.1016/j.nlm.2022.107649
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发表时间：
2022
影响因子：
2.7
通讯作者：
Disterhoft,JohnF
中科院分区：
文献类型：
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作者：
Rapp,AmyP;Hark,TimothyJ;Power,JohnM;Savas,JefferyN;MatthewOh,M;Disterhoft,JohnF
神经科学技术，包括体内记录，已经允许知识的极大扩展；然而，这项技术也可能影响研究人员着手调查的现象。我们将雌性和雄性小鼠纳入联想学习实验，揭示了慢性植入四极体对学习影响的性别差异。虽然先前的研究表明，完整的雌性小鼠比雄性和去卵巢的雌性小鼠更快地获得了微量的眨眼条件反射，但植入慢性微驱动器阵列对学习表现出性别二态效应。植入微驱动器的雄性小鼠比未植入微驱动器的雌性和去卵巢的雌性小鼠更快地获得联想学习范式。这些影响并不仅仅是因为驱动器的重量，因为接受“虚拟驱动器”植入而没有将四极电极植入大脑的动物在学习方面没有显著的性别差异。串联质谱标记质谱和western blot分析表明，MAPK通路、急性炎症和脑源性神经营养因子的显著改变可能是这些观察到的性别和手术依赖性学习影响的基础。
Neuroscience techniques, including in vivo recording, have allowed for a great expansion in knowledge; however, this technology may also affect the very phenomena researchers set out to investigate. Including both female and male mice in our associative learning experiments shed light on sex differences on the impact of chronic implantation of tetrodes on learning. While previous research showed intact female mice acquired trace eyeblink conditioning faster than male and ovariectomized females, implantation of chronic microdrive arrays showed sexually dimorphic effects on learning. Microdrive implanted male mice acquired the associative learning paradigm faster than both intact and ovariectomized females. These effects were not due to the weight of the drive alone, as there were no significant sex-differences in learning of animals that received “dummy drive” implants without tetrodes lowered into the brain. Tandem mass tag mass spectrometry and western blot analysis suggest that significant alterations in the MAPK pathway, acute inflammation, and brain derived neurotrophic factor may underlie these observed sex- and surgery-dependent effects on learning.