Identification of Cis-DNA regions controlling Bmp4 expression during tooth morphogenesis in vivo
Identification of Cis-DNA regions controlling Bmp4 expression during tooth morphogenesis in vivo
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DOI：
10.1177/154405910208100103
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发表时间：
2002-01-01
影响因子：
7.6
通讯作者：
Harris, SE
中科院分区：
文献类型：
--
作者：
Feng, JQ;Zhang, J;Harris, SE
牙齿形成需要上皮-间充质相互作用。骨形态发生蛋白4（Bmp 4）是这一过程中的关键信号分子。为了更好地了解Bmp 4基因在牙齿发育过程中的作用，我们研究了在发育过程中控制其时空表达的机制。使用转基因的方法，我们确定的结构域控制上皮来源的成釉细胞中的Bmp 4表达位于0.26 kb和1.1 kb的小鼠Bmp 4启动子之间的区域。相反，控制间充质源性成牙本质细胞和牙髓细胞中Bmp 4表达的结构域存在于Bmp 4基因的其他区域。我们还证明了控制Bmp 4在原始牙齿细胞中表达的结构域不同于控制Bmp 4在成熟牙齿组织中表达的结构域。通过控制Bmp 4基因的表达来确定独特的结构域，表明不同的转录因子在牙齿形态发生的不同阶段调节Bmp 4水平。
Epithelial-mesenchymal interactions are required for tooth formation. Bone morphogenetic protein 4 (Bmp4) is a crucial signaling molecule during this process. For better understanding of the role of the Bmp4 gene during tooth development, we studied the mechanisms that control its temporal and spatial expression during development. Using a transgenic approach, we determined that the domains which controlled Bmp4 expression in epithelium-derived ameloblasts were located in the region between 0.26 kb and 1.1 kb of the murine Bmp4 promoter. In contrast, the domains controlling Bmp4 expression in mesenchyme-derived odontoblasts and pulp cells existed in other regions of the Bmp4 gene. We have also demonstrated that the domains controlling Bmp4 expression in primordial tooth cells differ from those controlling Bmp4 expression in mature tooth tissues. The determination of unique domains by controlling the expression of the Bmp4 gene suggests that different transcriptional factors regulate the Bmp4 level at different stages during tooth morphogenesis.