Influence of tetraalkyl ammonium ions on the structure of poly (rG-dC).poly (rG-dC): unexpected transitions among the Z, A and B conformations.
Influence of tetraalkyl ammonium ions on the structure of poly (rG-dC).poly (rG-dC): unexpected transitions among the Z, A and B conformations.
复制标题
四烷基铵离子对聚 (rG-dC).聚 (rG-dC) 结构的影响：Z、A 和 B 构象之间的意外转变。
DOI：
10.1093/nar/15.9.3907
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发表时间：
1987
影响因子：
14.9
通讯作者：
Behe,MJ
中科院分区：
文献类型：
--
作者：
Jayasena,SD;Behe,MJ
在四烷基铵离子存在下，研究了双链混合核糖-脱氧核糖多核苷酸poly（rG-dC）·poly（rG-dC）的构象。四甲基铵离子稳定聚合物的“低盐”Z构象（1），从亚毫摩尔到摩尔浓度的反离子。在四乙基和四丙基铵离子的存在下，聚合物以低盐Z形式存在，直到2 mM浓度的抗衡离子，然后翻转为右手螺旋A形式。使用四丁基铵抗衡离子，聚合物在低离子浓度下呈A构象，并且在大于30毫摩尔的浓度下转化为B形式。这些结果被解释在静电和溶剂的多核苷酸的相互作用方面。
The conformation of the double-stranded, mixed ribo-deoxyribo polynucleotide, poly (rG-dC)•poly (rG-dC), has been examined in the presence of tetraalkyl ammonium ions. Tetramethyl ammonium ion stabilizes the “low salt” Z conformation (1) of the polymer from submillimolar to molar concentrations of the counterion. In the presence of tetraethyl and tetrapropyl ammonium ions the polymer exists in the low salt Z form up to 2 mM concentration of the counterions and then flips to the right hand helical A form. With tetrabutyl ammonium counterions the polymer is in an A conformation at low ion concentrations and converts to a B form at concentrations greater than thirty millimolar. These results are interpreted in terms of electrostatic and solvent interactions of the polynucleotide.