Microscopic observations and inflammatory cytokine productions of human macrophage phagocytising submicron titanium particles
Microscopic observations and inflammatory cytokine productions of human macrophage phagocytising submicron titanium particles
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人巨噬细胞吞噬亚微米钛颗粒的显微观察及炎性细胞因子的产生
DOI：
10.1007/s10856-009-3834-x
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
他4名
中科院分区：
文献类型：
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作者：
Taira;M.;Kagiya;T.;Harada;H.;Sasaki;M.;他4名
本研究通过显微观察和测量人巨噬细胞吞噬亚微米钛（Ti）颗粒产生的炎性细胞因子。二次电镜（SEM）、电镜/电子探针显微分析（EPMA）和透射电镜（TEM）观察表明，巨噬细胞[phorboll -12- myri酸-13-乙酸酯（PMA）分化的THP-1细胞]在培养24 h后积极吞噬并在细胞内吞噬体中积累亚微米Ti颗粒，并发生Ti颗粒的细化。巨噬细胞在含和不含亚微米钛颗粒和脂多糖（LPS；细菌成分）的四种培养基中培养24小时。虽然这两种刺激都没有降低细胞活力，但亚微米Ti颗粒和LPS激活单独和协同作用导致巨噬细胞在培养上清液中产生高水平的三种炎症细胞因子（TNF-α， IL-1β和IL-6）。巨噬细胞吞噬亚微米Ti颗粒引起的炎症和骨溶解情况会因LPS的攻击而恶化。
This study was performed to microscopically observe and measure inflammatory cytokine production by human macrophages phagocytosing submicron titanium (Ti) particles. Observations with secondary electron microscopy (SEM), SEM/electron probe microanalysis (EPMA) and transmission electron microscopy (TEM) indicated that macrophages [phorbol-12-myristate-13-acetate (PMA)-differentiated THP-1 cells] at 24 h in culture actively phagocytosed and accumulated submicron Ti particles in intracellular phagosomes, in which refinement of Ti particles occurred. The macrophages were also cultured for 24 h in four media with and without submicron Ti particles and lipopolysaccharide (LPS; components of bacteria). Whilst neither stimulus reduced cell viability, submicron Ti particles and LPS activation independently and synergistically caused the macrophages to produce three inflammatory cytokines (TNF-α, IL-1β and IL-6) at high levels in the culture supernatants. The inflammatory and osteolysis conditions caused by macrophages phagocytosing submicron Ti particles would be worsened by challenge with LPS in patients wearing Ti prostheses.