A 3RD DEFECTIVE LAMBDOID PROPHAGE OF ESCHERICHIA-COLI-K12 DEFINED BY THE LAMBDA-DERIVATIVE, LAMBDA-QIN111
A 3RD DEFECTIVE LAMBDOID PROPHAGE OF ESCHERICHIA-COLI-K12 DEFINED BY THE LAMBDA-DERIVATIVE, LAMBDA-QIN111
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DOI：
10.1016/s0022-2836(83)80124-7
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发表时间：
1983-01-01
影响因子：
5.6
通讯作者：
DAMBLYCHAUDIERE, C
中科院分区：
文献类型：
--
作者：
ESPION, D;KAISER, K;DAMBLYCHAUDIERE, C
一种新的Q-非依赖性置换突变体的分离和表征，Qin 111，它不同于其它特征的Q独立的Qin。，描述。这个变种人定义了一个新的...如细菌染色体中的原噬菌体，如通过大肠杆菌qin 111和宿主DNA之间的同源重组和通过DNA/DNA杂交方法所见。遗传和EM数据显示，这种新的原噬菌体至少携带类似于大肠杆菌Q-S-R的基因。以及在功能上与. cos相同的cos站点。cos.它位于34分钟的E。coliK 12图谱，即与缺陷型Rac原噬菌体在同一区域但在不同位点。
The isolation and characterization of a new Q-independent substitution mutant of .lambda., .lambda.qin111, which differs from other characterized Q-independent .lambda. phages, are described. This mutant defines a new .lambda.-like prophage in the bacterial chromosome, as seen by homologous recombination between .lambda.qin111 and the host DNA and by DNA/DNA hybridization methods. Genetic and EM data show that this new prophage carries, at least, genes analogous to Q-S-R of .lambda. and also a cos site functionally identical to .lambda. cos. It is located near 34 min on the E. coli K12 map, i.e, in the same region but at a different site from the defective Rac prophage.