13-fold resolution gain through turbid layer via translated unknown speckle illumination
13-fold resolution gain through turbid layer via translated unknown speckle illumination
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DOI：
10.1364/boe.9.000260
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发表时间：
2018-01-01
影响因子：
3.4
通讯作者：
Zheng, Guoan
中科院分区：
文献类型：
--
作者：
Guo, Kaikai;Zhang, Zibang;Zheng, Guoan
通过混浊层的荧光成像在各种生物光子学应用中具有很大的前景。传统的波前成形技术旨在通过混浊层产生和扫描聚焦光斑。在不直接进入目标平面的情况下找到正确的输入波前仍然是一个关键的挑战。在本文中，我们探索了一种新的策略，通过混浊层的大视场成像。在我们的设置中，荧光样品夹在两个混浊层之间。而不是通过波前整形产生一个焦点，我们使用一个未成形的光束照射混浊层，并产生一个未知的散斑图案在目标平面上的宽视场。通过倾斜输入波前，利用记忆效应对未知散斑进行光栅扫描，并通过混浊层捕获相应的低分辨率荧光图像。与基于波前整形的单点扫描不同，所提出的方法采用多个点（即，散斑）以平行地延伸视场。基于所有捕获的图像，我们共同恢复荧光对象，未知的混浊层的光学传递函数，平移步长，和未知的散斑图案。没有直接访问的对象平面或知识的混浊层，我们证明了13倍的分辨率增益通过混浊层使用报告的策略。我们还演示了使用这种技术来提高低数值孔径物镜透镜的分辨率，允许同时获得大视场和高分辨率。报道的方法为开发新的荧光成像平台提供了见解，并可能在深层组织成像中找到应用。(c)根据OSA开放获取出版协议的条款，2017年美国光学学会
Fluorescence imaging through a turbid layer holds great promise for various biophotonics applications. Conventional wavefront shaping techniques aim to create and scan a focus spot through the turbid layer. Finding the correct input wavefront without direct access to the target plane remains a critical challenge. In this paper, we explore a new strategy for imaging through turbid layer with a large field of view. In our setup, a fluorescence sample is sandwiched between two turbid layers. Instead of generating one focus spot via wavefront shaping, we use an unshaped beam to illuminate the turbid layer and generate an unknown speckle pattern at the target plane over a wide field of view. By tilting the input wavefront, we raster scan the unknown speckle pattern via the memory effect and capture the corresponding low-resolution fluorescence images through the turbid layer. Different from the wavefront-shaping-based single-spot scanning, the proposed approach employs many spots (i.e., speckles) in parallel for extending the field of view. Based on all captured images, we jointly recover the fluorescence object, the unknown optical transfer function of the turbid layer, the translated step size, and the unknown speckle pattern. Without direct access to the object plane or knowledge of the turbid layer, we demonstrate a 13-fold resolution gain through the turbid layer using the reported strategy. We also demonstrate the use of this technique to improve the resolution of a low numerical aperture objective lens allowing to obtain both large field of view and high resolution at the same time. The reported method provides insight for developing new fluorescence imaging platforms and may find applications in deep-tissue imaging. (c) 2017 Optical Society of America under the terms of the OSA Open Access Publishing Agreement