Whole berries versus berry anthocyanins: Interactions with dietary fat levels in the C57BL/6J mouse model of obesity
Whole berries versus berry anthocyanins: Interactions with dietary fat levels in the C57BL/6J mouse model of obesity
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DOI：
10.1021/jf071993o
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发表时间：
2008-02-13
影响因子：
6.1
通讯作者：
Howard, Luke R.
中科院分区：
文献类型：
--
作者：
Prior, Ronald L.;Wu, Xianli;Howard, Luke R.
雄性C57BL/6J小鼠分别接受92天(实验1)或70天(实验2)的高脂饮食[45%(HF45)或60%(HF60)脂肪来源的卡路里]，其中10%热量来自脂肪(LF)。这些试验使用或不使用来自整个蓝莓(BB)或草莓(SB)的冻干粉(Ext 1)或来自BB或SB的纯化花青素提取物(Ext 2)。使用Echo MRI确定身体成分。添加到LF饮食中的浆果不会改变体重增加、最终体重、体脂或蛋白质(体重百分比)或饮食(克)或能量(千卡)的摄入量。然而，在喂养HF45和HF60的小鼠中，与LF小鼠相比，体重增加、最终体重、体脂(百分比)和附睾脂重量增加，而身体蛋白质减少(p<；0.01)。在饲喂HF45饲料加BB的小鼠中，体重增长、体脂(占体重的百分比)和附睾脂重量显著高于饲喂HF45的对照组，而服用SB HF的小鼠的体重与HF对照组相似。SB或BB喂养并不改变糖耐量，尽管糖耐量随着年龄的增长而下降，HF45与LF小鼠的糖耐量相比有所下降。饲喂SB和HF45的小鼠的基线血糖较低。8周后，喂食HF60饲料加从BB中提纯的花青素的小鼠的体重增长和体脂低于喂食HF60的对照组。作为整个蓝莓喂养的花青素并不能预防肥胖，实际上可能会增加肥胖。然而，喂食从蓝莓或草莓中提纯的花青素可以减少肥胖。
Male C57BL/6J mice received diets with either 10% of calories from fat (LF) or a high-fat diet [45% (HF45) or 60% (HF60) calories from fat] for 92 days (expt 1) or 70 days (expt 2). These were given with or without freeze-dried powders from whole blueberries (BB) or strawberries (SB) (expt 1) or purified anthocyanin extracts from BB or SB (expt 2). Body composition was determined utilizing Echo MRI. Berries added to the LF diet did not alter weight gain, final body weights, body fat, or protein (percent body weight) or diet (grams) or energy (kilocalories) intake. However, in both HF45-and HF60-fed mice, weight gain, final weights, body fat (percent), and epididymal fat weights increased and body protein decreased (p < 0.01) compared to LF mice. In mice fed HF45 diet plus BB, body weight gains, body fat (percent of BW), and epididymal fat weights were significantly greater than those in the HF45-fed controls, whereas weights of mice fed SB HF were similar to those of HF controls. SB or BB feeding did not alter glucose tolerance, although glucose tolerance decreased with age and in HF45 versus LF mice. Baseline plasma glucose was lower in SB- versus HF45-fed mice. After 8 weeks, mice fed the HF60 diet plus purified anthocyanins from BB in the drinking water had lower body weight gains and body fat than the HF60-fed controls. Anthocyanins fed as the whole blueberry did not prevent and may have actually increased obesity. However, feeding purified anthocyanins from blueberries or strawberries reduced obesity.