TREPONEMA-PALLIDUM INVADES INTERCELLULAR-JUNCTIONS OF ENDOTHELIAL-CELL MONOLAYERS
TREPONEMA-PALLIDUM INVADES INTERCELLULAR-JUNCTIONS OF ENDOTHELIAL-CELL MONOLAYERS
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DOI：
10.1073/pnas.85.10.3608
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发表时间：
1988-05-01
影响因子：
11.1
通讯作者：
LOVETT, MA
中科院分区：
文献类型：
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作者：
THOMAS, DD;NAVAB, M;LOVETT, MA
梅毒的发病机制反映了梅毒螺旋体的侵袭特性，但实际的组织侵袭方式尚不清楚。我们在体外发现了密螺旋体侵袭性的两个相似之处。我们测试了运动T。梅毒螺旋体可以侵入宿主细胞，通过确定放射性标记的能动生物体的命运添加到HeLa细胞单层; 26%的密螺旋体与单层在胰蛋白酶抗性龛，大概之间的单层和表面，它坚持，但没有达到胞内。T的附着。pallidum对培养的人兔主动脉和人脐静脉内皮细胞的作用是对HeLa细胞的2倍。我们添加了T。在细胞间紧密连接形成的条件下，在膜滤器上生长的主动脉内皮细胞。T.通过电阻测量，苍白球能够穿过内皮细胞单层而不改变紧密连接。热致死T.梅毒螺旋体和非病原体吞噬密螺旋体生物型Reiter未能穿透单层。单层切片的透射电子显微镜照片显示T。苍白球在细胞间连接。我们在体外的观察表明，这些高度能动的螺旋体可能会离开循环侵入内皮细胞之间的连接。
The pathogenesis of syphilis reflects invasive properties of Treponema pallidum, but the actual mode of tissue invasion is unknown. We have found two in vitro parallels of treponemal invasiveness. We tested whether motile T. pallidum could invade host cells by determining the fate of radiolabeled motile organisms added to a HeLa cell monolayer; 26% of treponemes associated with the monolayer in a trypsin-resistant niche, presumably between the monolayer and the surface to which it adhered, but did not attain intracellularity. Attachment of T. pallidum to cultured human rabbit aortic and human umbilical vein endothelial cells was 2-fold greater than to HeLa cells. We added T. pallidum to aortic endothelial cells grown on membrane filters under conditions in which tight intercellular junctions had formed. T. pallidum was able to pass through the endothelial cell monolayers without altering tight junctions, as measured by electrical resistance. In contrast, heat-killed T. pallidum and the nonpathogen Treponema phagedenis biotype Reiter failed to penetrate the monolayer. Transmission electron micrographs of sections of the monolayer showed T. pallidum in intercellular junctions. Our in vitro observations suggest that these highly motile spirochetes may leave the circulation by invading the junctions between endothelial cells.