Diagnostic Potential of IgG and IgA Responses toMycobacterium tuberculosisAntigens for Discrimination among Active Tuberculosis, Latent Tuberculosis Infection, and Non-Infected Individuals
Diagnostic Potential of IgG and IgA Responses toMycobacterium tuberculosisAntigens for Discrimination among Active Tuberculosis, Latent Tuberculosis Infection, and Non-Infected Individuals
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DOI：
10.3390/microorganisms8070979
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发表时间：
2020-07-01
期刊：
影响因子：
4.5
通讯作者：
Kim, Bum-Joon
中科院分区：
文献类型：
--
作者：
Lee, Ji Yeon;Kim, Byoung-Jun;Kim, Bum-Joon
结核病仍然是一个主要的公共卫生问题。传统检测不足以区分活动性结核病（ATB）和潜伏性结核感染（LTBI）。我们测量了 ATB、LTBI 和非感染 (NI) 个体对结核分枝杆菌抗原（结核分枝杆菌分支酸变位酶 (TBCM)、抗原 85B (Ag85B)、早期分泌抗原 6 (ESAT-6) 和培养物滤过蛋白 10 (CFP-10) 的抗体反应。血清免疫球蛋白 G (IgG) 和免疫球蛋白 A (IgA) 水平为测量并使用 QuantiFERON-TB Gold In-Tube 检测来诊断 LTBI。LTBI 受试者的 IgG 水平显着高于 NI 受试者，ATB 受试者的抗 Ag85B IgG 水平和抗 CFP-10 的 IgG 水平显着较高，其次是 LTBI，然后是 NI。 LTBI 和 NI 组中观察到 LTBI 和 NI 受试者的干扰素 γ 和抗 Ag85B、TBCM 和 CFP-10 的 IgG 水平以及抗 Ag85B 的 IgA 水平呈正相关趋势，表明抗 TBCM 和 CFP-10 的 IgG 与 LTBI 的诊断独立相关，抗 Ag85B 的 IgG 与 ATB 的诊断独立相关，并且可以区分 LTBI 和 ATB。总体而言，IgG 抗体对 TBCM、Ag85B 和 CFP-10 的反应可以区分 ATB、LTBI 和 NI 组。
Tuberculosis remains a major public health problem. Conventional tests are inadequate to distinguish between active tuberculosis (ATB) and latent tuberculosis infection (LTBI). We measured antibody responses toMycobacterium tuberculosisantigens (Mycobacterium tuberculosischorismate mutase (TBCM), antigen 85B (Ag85B), early secreted antigen-6 (ESAT-6), and culture filtrate protein-10 (CFP-10) in ATB, LTBI, and non-infected (NI) individuals. Serum immunoglobulin G (IgG) and immunoglobulin A (IgA) levels were measured and the QuantiFERON-TB Gold In-Tube assay was used to diagnose LTBI. IgG levels against TBCM were significantly higher in LTBI than NI subjects. IgG and IgA levels against Ag85B and IgG levels against CFP-10 were significantly higher in ATB, followed by LTBI, and then NI. When the ATB group was subdivided, IgG levels against Ag85B and CFP-10 were significantly higher in each subgroup compared with those in LTBI and NI groups. Positive correlation trends between interferon-gamma and IgG levels against Ag85B, TBCM, and CFP-10 and IgA levels against Ag85B in LTBI and NI subjects were observed. Age- and sex-adjusted models showed that IgG against TBCM and CFP-10 was independently related to LTBI diagnosis, and IgG against Ag85B was independently related to the diagnosis of ATB and could distinguish between LTBI and ATB. Overall, IgG antibody responses to TBCM, Ag85B, and CFP-10 can discriminate among ATB, LTBI, and NI groups.