LIGHT SCATTERING AND BIREFRINGENCE CHANGES DURING ACTIVITY IN ELECTRIC ORGAN OF ELECTROPHORUS ELECTRICUS
LIGHT SCATTERING AND BIREFRINGENCE CHANGES DURING ACTIVITY IN ELECTRIC ORGAN OF ELECTROPHORUS ELECTRICUS
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DOI：
10.1113/jphysiol.1969.sp008876
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发表时间：
1969-01-01
影响因子：
5.5
通讯作者：
KEYNES, RD
中科院分区：
文献类型：
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作者：
COHEN, LB;HILLE, B;KEYNES, RD
1.为了获得与电活性有关的结构变化的信息，我们已经确定了在电器官放电期间和之后发生的光散射和生物频率变化的大小和时间过程。对于单次放电，光电倍增管检测到的光强度变化从未超过0.2%，并且通常比这小得多，但通过信号平均技术可以获得具有可接受的信噪比的记录。3.一个单一的刺激导致减少，然后增加，最后又减少的光散射切片的主要电器官。这三个阶段被指定为E1、E2和E3.4。E1开始于动作电位的开始，其峰值与复极化完成同时达到，即使复极化因冷却而延迟或因从组织中抽取较大电流而加速. E2与流过电器官切片的电流的积分成正比，并且可能是由镶嵌在极板表面的小管的膨胀和收缩引起的。它在施加电流开始的一两毫秒内发展，并持续约100毫秒。E3是持续几秒钟的散射的可变减少。7.刺激也会导致电器官的双折射暂时增加。光学变化跟随着电极板神经支配面电位的变化，延迟略低于50 μ sec。双折射的这种电压依赖性变化可能是由膜中的克尔效应（电双折射）或膜的压缩引起的。
1. In an attempt to obtain information about structural changes related to electrical activity inElectrophoruselectroplates, we have determined the size and time course of the changes in light scattering and in bire‐fringence that occur during and after the discharge of the electric organ.2. The changes in light intensity detected with a photomultiplier were never greater than 0·2% for a single discharge, and were often much smaller than this, but records with an acceptable ratio of signal to noise could be obtained by signal‐averaging techniques.3. A single stimulus led to a decrease, then an increase, and finally another decrease in the light scattered by slices of the main electric organ. These three phases were designated E1, E2 and E3.4. E1 started at the beginning of the action potential, and its peak was reached at the same time as the completion of repolarization, even when the repolarization was delayed by cooling or hastened by drawing larger currents from the tissue.5. E2 was proportional to the integral of the current flowing through the slice of electric organ, and may arise from the swelling and shrinking of the tubules that stud the faces of the electroplates. It developed within a millisecond or two of the start of an applied current, and lasted for about 100 msec.6. E3 was a variable decrease in scattering that lasted for some seconds.7. A stimulus also led to a transient increase in the birefringence of the electric organ. The optical change followed the change in electrical potential across the innervated faces of the electroplates with a delay of somewhat under 50 μsec.8. This voltage‐dependent change in birefringence may arise from a Kerr effect (electric birefringence) in the membrane or from compression of the membrane.