Neuropeptide precursor gene discovery in the Chagas disease vector Rhodnius prolixus
Neuropeptide precursor gene discovery in the Chagas disease vector Rhodnius prolixus
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DOI：
10.1111/j.1365-2583.2010.01050.x
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发表时间：
2011-02-01
影响因子：
2.6
通讯作者：
Rivera-Pomar, R.
中科院分区：
文献类型：
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作者：
Ons, S.;Sterkel, M.;Rivera-Pomar, R.
我们展示了一个简单的工作流程，结合同源性搜索Rhodnius prolixus基因组序列与克隆的cDNA末端的快速扩增和质谱。我们已经确定了32个基因和它们的转录本，编码一些神经肽前体，导致194个推定的肽。我们用质谱法验证了82个预测的神经肽在R。prolixus实现了第一个全面的基因组，转录组和神经肽组分析的昆虫疾病载体。比较现有的昆虫神经肽序列显示，R。prolixus基因组包含了昆虫中大部分保守的神经肽，其中许多在序列水平上具有特异性。本文报道的一些基因家族首次在半翅目昆虫中被鉴定，半翅目昆虫是一个高度生物多样性的昆虫类群，包括许多人类、动物和植物疾病病原体。
We show a straightforward workflow combining homology search in Rhodnius prolixus genome sequence with cloning by rapid amplification of cDNA ends and mass spectrometry. We have identified 32 genes and their transcripts that encode a number of neuropeptide precursors leading to 194 putative peptides. We validated by mass spectrometry 82 of those predicted neuropeptides in the brain of R. prolixus to achieve the first comprehensive genomic, transcriptomic and neuropeptidomic analysis of an insect disease vector. Comparisons of available insect neuropeptide sequences revealed that the R. prolixus genome contains most of the conserved neuropeptides in insects, many of them displaying specific features at the sequence level. Some gene families reported here are identified for the first time in the order Hemiptera, a highly biodiverse group of insects that includes many human, animal and plant disease agents.