The structure of a gene containing introns and encoding rat ribosomal protein P2.
The structure of a gene containing introns and encoding rat ribosomal protein P2.
复制标题
含有内含子并编码大鼠核糖体蛋白 P2 的基因的结构。
DOI：
10.1093/nar/19.18.4895
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发表时间：
1991
影响因子：
14.9
通讯作者：
Wool,IG
中科院分区：
文献类型：
--
作者：
Chan,YL;Wool,IG
含有内含子的单个大鼠核糖体蛋白 P2 基因已得到表征。它有2275个核苷酸，分布在5个外显子和4个内含子中。外显子中编码的氨基酸序列与之前从 cDNA 衍生的氨基酸序列完全一致。在大约 9 个成员的家族中，只有这一个 P2 基因具有内含子并被表达。有两个转录起始位点（相邻的胞苷残基）位于 10 个嘧啶序列中，两侧是富含 GC 的区域。 P2基因与其他哺乳动物核糖体蛋白基因一样，缺乏TATA盒；然而，它在位置-30至-27处具有序列TTTA，其可能是简并的TATA框并且可以起到相同的功能。 P2 基因的结构，特别是启动子区域的结构，与其他哺乳动物核糖体蛋白基因的结构相似。这表明共同特征有助于其转录的协调调节，并且 P2（其在核糖体中以 2 个拷贝存在）的化学计量是通过调节其 mRNA 的翻译来实现的。
The single rat ribosomal protein P2 gene containing introns has been characterized. It has 2275 nucleotldes distributed in 5 exons and 4 introns. The sequence of amino acids encoded in the exons corresponds exactly to that derived before from a cDNA. Only this one P2 gene in a family of approximately 9 members has introns and is expressed. There are two transcriptional start sites (adjacent cytidine residues) located in a tract of 10 pyrimldlnes flanked by GC-rich regions. The P2 gene, like other mammalian ribosomal protein genes, lacks a TATA box; however, it has at positions −30 to −27 the sequence TTTA which may be a degenerate TATA box and may serve the same function. The architecture of the P2 gene, and especially the structure of the promoter region, resembles that of other mammalian ribosomal protein genes. This suggests that the common features contribute to the coordinate regulation of their transcription and that the stoichiometry of P2 (it is present in 2 copies in the ribosome) is achieved by regulation of the translation of Its mRNA.