Digital quantification of precursor frequency in the fallopian tube and its significance
Digital quantification of precursor frequency in the fallopian tube and its significance
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DOI：
10.1038/modpathol.2012.100
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发表时间：
2012-12-01
期刊：
影响因子：
7.5
通讯作者：
Crum, Christopher P.
中科院分区：
文献类型：
--
作者：
Bijron, Jonathan G.;Ning, Gang;Crum, Christopher P.
高频率的前驱病变是许多器官系统癌症的危险因素，但必须精确量化。盆腔浆液性肿瘤与输卵管中分泌细胞增生 (SCOUT) 频率增加以及 PAX2 蛋白 (PAX2p) 表达缺失 (PAX2p-null SCOUT) 相关。然而，为了证实这一点，必须相对于上皮表面精确量化 PAX2p-null SCOUT。我们开发了一种方法，使用 iScan 明场扫描仪 (BioImagene) 对输卵管切片进行数字化，并上传到 Adobe Photoshop CS3 Extended。像素长度被转换为微米，上皮长度用魔棒工具测量。 SCOUT 表示为总上皮周长的函数。确定了频率、所需周长、地形聚集趋势和年龄的影响。病例每 10cm 的 SCOUT 频率为 0-4.60，对照组为 0-1.66，平均值分别为 0.84 和 0.27（P = 0.007）。在浆液性癌症病例中，SCOUT 检测所需的周长较短，并且拓扑分布遵循随机模式，没有异常聚类。年龄也与 SCOUT 频率相关（P = 0.025），并且在调整年龄后，癌症和对照之间的差异仍然显着（P = 0.001）。我们描述了一种量化输卵管上皮周长的有效方法，并验证其与前体频率的相关性。这对于评估输卵管和其他器官（例如前列腺、胰腺和结肠）中的前体细胞频率具有重要意义，在这些器官中，上皮前体细胞是癌发生不可或缺的一部分。现代病理学(2012) 25, 1654-1661; doi:10.1038/modpathol.2012.100; 2012 年 7 月 6 日在线发布
A high frequency of precursor lesions is a risk factor for cancer in many organ systems but must be precisely quantified. Pelvic serous neoplasia is associated with an estimated increase in frequency of secretory cell outgrowths (SCOUTs) with loss of PAX2 protein (PAX2p) expression (PAX2p-null SCOUTs) in the fallopian tube. However, to confirm this, PAX2p-null SCOUTs must be precisely quantified relative to the epithelial surface. We developed a method by which fallopian tube sections were digitized using an iScan brightfield scanner (BioImagene) and uploaded in Adobe Photoshop CS3 Extended. Pixel length was translated into microns and epithelial length measured with the Magic Wand tool. SCOUTs were expressed as a function of total epithelial perimeter. Frequency, required perimeter length, topographic clustering tendency and effects of age were ascertained. SCOUT frequency per 10cm was 0-4.60 for cases and 0-1.66 for controls, averaging 0.84 and 0.27, respectively, (P = 0.007). Required perimeter length for SCOUT detection was less in serous cancer cases and topographic distribution followed a random pattern without aberrant clustering. Age was also associated with SCOUT frequency (P = 0.025) and differences between cancers and controls were still significant after adjusting for age (P = 0.001). We describe an efficient method for quantifying epithelial perimeter in the fallopian tube and verify its relevance to precursor frequency. This has important implications for assessing precursor frequency both in the fallopian tube and in other organs-such as prostate, pancreas and colon-where epithelial precursors are integral to carcinogenesis. Modern Pathology (2012) 25, 1654-1661; doi:10.1038/modpathol.2012.100; published online 6 July 2012