Detection of Rickettsia parkeri from within Piura, Peru, and the First Reported Presence of Candidatus Rickettsia andeanae in the Tick Rhipicephalus sanguineus
Detection of Rickettsia parkeri from within Piura, Peru, and the First Reported Presence of Candidatus Rickettsia andeanae in the Tick Rhipicephalus sanguineus
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DOI：
10.1089/vbz.2012.1028
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发表时间：
2013-07-01
影响因子：
2.1
通讯作者：
Richards, Allen L.
中科院分区：
文献类型：
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作者：
Flores-Mendoza, Carmen;Florin, David;Richards, Allen L.
2008年3月，在秘鲁西北部对家畜(n=145)进行了体表寄生虫的抽样。90只家畜(62%)检出体外寄生虫(S)，共检出寄生虫728只[斑点钝线虫、无中心蚤、微小牛蚤、血缘管头蚤和梅尼尼管蚤]，12只虱子(猪血吸虫)和3只蚤类(猫蚤)。对采集的体外寄生虫进行立克次体属特异性定量聚合酶链式反应，检出5%的体外寄生虫阳性(37/743，35只硬蜱和2只蚤)。用另外两种定量聚合酶链式反应检测阳性个体的DNA，以检测念珠菌或帕氏立克次体中ompB基因的存在。在25只斑点姬鼠和2只Rh.在6只斑点硬蜱中检出帕氏弧菌。两株斑点拟青霉同时感染安氏假丝酵母和帕氏假丝酵母菌。通过17-kD抗原和OmpA基因的多位点序列分型，在2只猫头幼蚤中检出了猫立克次体。这些发现将帕氏假丝酵母菌的地理范围扩大到包括秘鲁，并将假丝酵母菌的自然节肢动物媒介扩大到包括血缘轮虫。
Domestic farm animals (n = 145) were sampled for the presence of ectoparasites in northwestern Peru during March, 2008. Ninety domestic animals (62%) were positive for the presence of an ectoparasite(s) and produced a total collection of the following: 728 ticks [Amblyomma maculatum, Anocentor nitens, Rhipicephalus (Boophilus) microplus, Rhipicephalus sanguineus, and Otobius megnini], 12 lice (Haematopinus suis), and 3 fleas (Ctenocephalides felis). A Rickettsia genus-specific qPCR assay was performed on nucleic acid preparations of the collected ectoparasites that resulted in 5% (37/743, 35 ticks and 2 fleas) of the ectoparasites positive for the presence of Rickettsia. DNA from the positive individual ticks was tested with 2 other qPCR assays for the presence of the ompB gene in Candidatus Rickettsia andeanae or Rickettsia parkeri. Candidatus R. andeanae was found in 25 A. maculatum ticks and in two Rh. sanguineus ticks, whereas R. parkeri was detected in 6 A. maculatum ticks. Two A. maculatum were co-infected with both Candidatus R. andeanae and R. parkeri. Rickettsia felis was detected in 2 fleas, Ctenocephalides felis, by multilocus sequence typing of the 17-kD antigen and ompA genes. These findings expand the geographic range of R. parkeri to include Peru as well as expand the natural arthropod vector of Candidatus R. andeanae to include Rhipicephalus sanguineus.