Cross-reactivity of human luteinizing hormone (beta-subunit) and a serine protease demonstrated by immunoperoxidase staining in human oocytes.
Cross-reactivity of human luteinizing hormone (beta-subunit) and a serine protease demonstrated by immunoperoxidase staining in human oocytes.
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通过人卵母细胞中的免疫过氧化物酶染色证明人黄体生成激素（β-亚基）和丝氨酸蛋白酶的交叉反应性。
作者：
G. Jagiello;R. Mesa
应用免疫组织化学技术，观察了人LH β亚基（hLHβ）在月经周期不同时期的人窦卵泡卵母细胞胞浆和胞核中的定位。在周期时间比在浪涌期间加上两个令人困惑的技术方面的本地化相当数量的LH的存在，促使进一步的研究的基础上与丝氨酸蛋白酶的交叉反应性建议由其他报告。通过预先用hLHβ（合成同源（CAGY）序列[H2 N-Thr- Gly-Tyr-His-NH 2]）吸收hLHβ抗血清，可完全消除染色反应。用正常人血浆吸收后，观察到几乎完全消除，而用胰凝乳蛋白酶原A吸收后，可部分阻断染色反应。采用大鼠和人垂体前叶切片染色法评价抗hLH β抗体与hLHβ的吸收完全性。性腺激素染色未观察到明显差异……
Localization of the β-subunit of human LH (hLHβ) in the cytoplasm and nucleus of human oocytes obtained from antral follicles at different times of the menstrual cycle was demonstrated by immunohistochemical techniques. The presence of considerable amounts of LH at cycle times other than during the surge coupled with two puzzling technical aspects of the localization prompted further study based on a cross-reactivity with serine proteases suggested by other reports. Complete elimination of the staining reaction was achieved by prior absorption of the hLHβ antiserum with hLHβ, the synthetic homologous (CAGY) sequence [H2N-Thr- Gly-Tyr-His-NH2].Almost total elimination was observed after absorption with normal human plasma, while partial blocking was achieved after absorption with chymotrypsinogen A. Staining of sections of rat and human anterior pituitary glands was used to evaluate the completeness of the absorption of antihLHβ with hLHβ. No appreciable difference was seen in the staining of the gonadotro...