Molecular cloning, characterization, and tissue distribution of rat lipopolysaccharide binding protein. Evidence for extrahepatic expression.
Molecular cloning, characterization, and tissue distribution of rat lipopolysaccharide binding protein. Evidence for extrahepatic expression.
复制标题
DOI：
10.4049/jimmunol.153.2.743
复制
发表时间：
1994-07
影响因子：
4.4
通讯作者：
G. Su;P. Freeswick;D. Geller;Qi Wang;R. Shapiro;Y. Wan;T. Billiar;D. Tweardy;R. L. Simmons
中科院分区：
文献类型：
--
作者：
G. Su;P. Freeswick;D. Geller;Qi Wang;R. Shapiro;Y. Wan;T. Billiar;D. Tweardy;R. L. Simmons
LPS 结合蛋白 (LBP) 是正常血清中存在的一种糖蛋白，在急性期反应期间会显着升高。据报道，枸杞多糖可以极大地增强宿主对内毒素或脂多糖的反应。因此，腰痛可能在身体对损伤和感染的反应中发挥关键作用。关于调节 LBP 产生的因素知之甚少。为了研究LBP表达的调控，我们克隆了大鼠LBP的全长cDNA。推测的大鼠腰痛的氨基酸序列与报道的兔和人腰痛的氨基酸序列高度同源。大鼠 LBP 的序列进一步完善了与 LPS 结合的蛋白质家族中发现的保守区域；该家族由多个物种的杀菌渗透性增加蛋白和 LBP 组成。使用大鼠 LBP cDNA 克隆进行 Northern 印迹分析表明，在急性期反应期间，肝脏中的 LBP mRNA 水平显着上调。然而，与之前的报道相反，我们还发现了在这些诱导条件下 LBP 肝外表达的证据。 LBP mRNA 在肝脏以外的活化组织中的存在表明，LBP 在局部组织对 LPS 的反应中发挥的作用可能比以前认为的更大。
LPS binding protein (LBP) is a glycoprotein present in normal serum that becomes markedly elevated during acute phase responses. LBP has been reported to greatly potentiate host responses to endotoxin or LPS. Therefore, LBP may play a critical role in the body's response to injury and infection. Little is known about the factors regulating production of LBP. To investigate the regulation of LBP expression, we have cloned the full-length cDNA for rat LBP. The deduced amino acid sequence of rat LBP was highly homologous with that reported for rabbit and human LBP. The sequence of rat LBP further refines the conserved regions found within the family of proteins that bind LPS; this family is comprised of bactericidal permeability-increasing protein and LBP from multiple species. Use of the rat LBP cDNA clone for Northern blot analysis reveals that LBP mRNA levels are markedly up-regulated in liver during acute phase responses. However, in contrast to previous reports, we also find evidence of extrahepatic expression of LBP under these induced conditions. The presence of LBP mRNA in activated tissues other than liver suggests that LBP may play a larger role in local tissue responses to LPS than previously appreciated.