Temperature- and cyclic nucleotide-induced phase transitions of Histoplasma capsulatum.
Temperature- and cyclic nucleotide-induced phase transitions of Histoplasma capsulatum.
复制标题
温度和环核苷酸诱导的荚膜组织胞浆菌相变。
DOI：
10.1128/jb.146.1.117-120.1981
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发表时间：
1981
影响因子：
3.2
通讯作者：
Medoff,G
中科院分区：
文献类型：
--
作者：
Sacco,M;Maresca,B;Kumar,BV;Kobayashi,GS;Medoff,G
从酵母到菌丝的转变可以通过将孵育温度从37℃转移到25℃来完成，同时伴随着细胞代谢的许多变化，包括呼吸的变化，细胞内环腺苷3′，5′-单磷酸（cAMP）水平的变化，以及酵母期特异性酶半胱氨酸还原酶（EC 1.6.4.1）和半胱氨酸氧化酶（EC 1.13.11.20）的活性的变化。即使在37℃的温度下，cAMP和提高细胞内cAMP水平的物质（茶碱、乙酰水杨酸、前列腺素E1和神经生长因子）也能诱导酵母向菌丝转变。在这个形态发生过程中，发生了与温度诱导转变相同的变化模式。因此，这些变化不是简单地依赖于温度的变化，而是相变过程的一部分。
The transition from yeast to mycelia of Histoplasma capsulatum could be accomplished by shifting the temperature of incubation from 37 to 25 degrees C. It was accompanied by many changes in cellular metabolism, including changes in respiration, intracellular cyclic adenosine 3',5'-monophosphate (cAMP) levels, and activities of two enzymes specific for the yeast phase, cystine reductase (EC 1.6.4.1) and cysteine oxidase (EC 1.13.11.20). Even at 37 degrees C, the yeast to mycelial transition could be induced by cAMP and agents which raise the intracellular levels of cAMP (theophylline, acetylsalicylic acid, prostaglandin E1, and nerve growth factor). During this morphogenesis the same pattern of changes occurred as in the temperature-induced transition. Therefore, these changes were not simply dependent on a shift in temperature, but rather were part of the process of the phase transition.