Antioxidative system in sweet potato rootis activated by low-temperature storage
Antioxidative system in sweet potato rootis activated by low-temperature storage
复制标题
低温贮藏激活甘薯根部抗氧化系统
DOI：
10.1002/jsfa.9604
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发表时间：
2019-06-01
影响因子：
4.1
通讯作者：
Zhang, Hua
中科院分区：
文献类型：
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作者：
Wang, Si-Qi;Tang, Jun;Zhang, Hua
背景甘薯在低温贮藏过程中易发生冷害。为探讨低温伤害与活性氧代谢的关系，以典型耐贮品种徐薯32和耐贮敏感品种盐薯25为材料，分析了活性氧含量、抗氧化酶活性及基因表达。结果在低温胁迫下，抗坏血酸过氧化物酶（APX）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽还原酶（GR）等抗氧化酶活性在贮藏初期迅速升高。此后，由于ROS清除酶的逐渐减少，ROS代谢产物的含量持续增加。耐低温品种徐薯32的抗氧化酶活性和基因表达量高于耐低温品种盐薯25，抗氧化代谢产物含量高于盐薯25，而活性氧代谢产物含量低于盐薯25，表明抗氧化酶和抗氧化剂清除活性氧的能力与甘薯耐低温性密切相关。结论甘薯贮藏根中抗氧化系统被激活，抗氧化能力与耐贮品种的耐贮性呈正相关。(c)2019化学工业协会
BACKGROUND Sweet potato is susceptible to chilling injury during low-temperature storage. To explore the correlation between chilling injury and reactive oxygen species (ROS) metabolism, the content of ROS and the activities and gene expression of antioxidant enzymes were analyzed in the typical storage-tolerant cultivar Xushu 32 and storage-sensitive cultivar Yanshu 25. RESULTS The activities of antioxidant enzymes including ascorbate peroxidase (APX), superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) were enhanced rapidly in the early period of storage in response to chilling stress. Thereafter, the content of ROS metabolites increased consistently due to gradual decrease in ROS scavenging enzymes. Storage-tolerant cultivar Xushu 32 had higher antioxidant enzyme activities and gene expressions as well as higher content of antioxidant metabolites and lower content of ROS metabolites compared with storage-sensitive cultivar Yanshu 25, suggesting that the capacity of ROS scavenging by antioxidant enzymes and antioxidants is highly associated with the tolerance of sweet potato to chilling stress. CONCLUSION These results indicated that the antioxidative system is activated in the storage root of sweet potato and the antioxidative capacity is positively associated with better storage performance in the storage-tolerant cultivar. (c) 2019 Society of Chemical Industry