Cadmium inducible Fe deficiency responses observed from macro and molecular views in tobacco plants
Cadmium inducible Fe deficiency responses observed from macro and molecular views in tobacco plants
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DOI：
10.1007/s00299-005-0092-3
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发表时间：
2006-04-01
影响因子：
6.2
通讯作者：
Goto, F
中科院分区：
文献类型：
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作者：
Yoshihara, T;Hodoshima, H;Goto, F
研究了烟草幼苗对Cd暴露的反应。使用宏观和分子指数。100亩MCd暴露降低了幼苗的总干重和叶绿素指数，与真缺铁的影响相当。镉暴露降低了地上部的铁浓度，而增加了根中的铁浓度，尤其是在质外区。Cd可能主要干扰了铁从根部向地上部的运转，使植株上部处于缺铁状态。外源和内源缺铁响应基因HvIDS2(PRO)：：GUS、NtFRO1和NtIRT1在Cd胁迫下表达增强。这是首次在分子水平上证明镉诱导的铁缺乏的反应。
Responses induced by Cd exposure were assessed in tobacco seedlings (Nicotiana tabacum L.) using macro and molecular indices. The 100 mu M of Cd exposure reduced the total dry weight and chlorophyll index of the seedlings as much as the genuine Fe-deficiency. Concentration of Fe in the shoots decreased, whereas that in the roots increased by the Cd exposure, especially in the apoplasmic space. It is probable that Cd interferes mainly with the step of Fe-translocation from the roots to shoots and this sets the upper-part of the plant in a state of Fe-deficiency. The Cd exposure coordinately increased the expressions of the exogenous and the endogenous Fe-deficiency responsive genes, HvIDS2 (pro) ::GUS, NtFRO1 and NtIRT1 in the roots. This is the first data to demonstrate the responses of Cd-inducible Fe-deficiency at a molecular level.