A PNA-based Lab-on-PCB diagnostic platform for rapid and high sensitivity DNA quantification
A PNA-based Lab-on-PCB diagnostic platform for rapid and high sensitivity DNA quantification
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DOI：
10.1016/j.bios.2018.09.006
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发表时间：
2019-01-01
影响因子：
12.6
通讯作者：
Moschou, Despina
中科院分区：
文献类型：
--
作者：
Jolly, Pawan;Rainbow, Joshua;Moschou, Despina
我们报告的开发实验室上PCB的DNA诊断平台，利用肽核酸（PNA）序列作为探针。该研究展示了两个商业PCB制造生物传感应用的金电镀工艺的优化和表征。使用PNA与间隔分子（MCH）的优化比例，迄今为止基于PCB的DNA生物传感器的最低检测限（LoD）为57 fM。该研究还展示了一个完全集成的PCB上实验室微系统，该系统专为快速检测而设计，采用PCB集成的样品输送，在连续流动下5分钟内分析5 μ L样品，实现0.1-100 pM范围内的DNA定量。所展示的生物传感器证明了基于PCB的DNA生物传感器的高灵敏度的能力，并为它们在PCB上实验室DNA诊断微系统中的集成铺平了道路。
We report the development of a Lab-on-PCB DNA diagnostic platform, exploiting peptide nucleic acid (PNA) sequences as probes. The study demonstrates the optimization and characterization of two commercial PCB manufacturing gold electroplating processes for biosensing applications. Using an optimized ratio of PNA with a spacer molecule (MCH), the lowest limit of detection (LoD) to date for PCB-based DNA biosensors of 57 fM is reported. The study also showcases a fully integrated Lab-on-PCB microsystem designed for rapid detection, which employs PCB-integrated sample delivery, achieving DNA quantification in the 0.1-100 pM range for 5 mu L samples analyzed within 5 min under continuous flow. The demonstrated biosensor proves the capability of PCB based DNA biosensors for high sensitivity and paves the way for their integration in Lab-on-PCB DNA diagnostic microsystems.