Telocytes: ultrastructural, immunohistochemical and electrophysiological characteristics in human myometrium.
Telocytes: ultrastructural, immunohistochemical and electrophysiological characteristics in human myometrium.
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DOI：
10.1530/rep-12-0369
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发表时间：
2013-04
期刊：
影响因子：
--
通讯作者：
Popescu LM
中科院分区：
文献类型：
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作者：
Cretoiu SM;Cretoiu D;Marin A;Radu BM;Popescu LM
在各种器官和物种中，端细胞（TC）被描述为具有端足（Tps）的细胞（www.telocytes.com）-具有交替的薄节（足节）和扩张部分（足节）的非常长的细胞延伸。我们使用电子显微镜（EM），免疫组织化学（IHC），免疫荧光（IF），延时视频显微镜和全细胞膜片钳检查TC。EM显示了一个三维网络的二叉分支的Tps，一个具有同源和异源细胞连接的细胞系统。TPS释放细胞外囊泡（非妊娠子宫肌层的平均直径为160.6±6.9 nm，妊娠子宫肌层的平均直径为171.6±4.6 nm），向邻近细胞发送大分子信号。 比较测量（非妊娠和妊娠子宫肌层）足突厚度的值为81.94±1.77 vs 75.53±1.81 nm，而足突直径为268.6±8.27 vs 316.38±17.56 nm。 免疫组化和免疫荧光检测显示c-kit和CD 34双阳性结果。细胞培养的延时视频显微镜显示Tps和肌细胞之间的动态相互作用。在非妊娠子宫肌层中，TC的膜片钳记录显示了一种超极化激活的氯离子内向电流，具有钙依赖性和L型钙通道的缺乏。TC似乎没有类似于周围平滑肌细胞（SMC）的兴奋特性。总之，本研究表明，TC作为一种不同的细胞类型在人类非妊娠和妊娠子宫肌层的存在，并描述了两种生理状态之间的形态差异。此外，我们提供了一个初步的体外电生理评估的非妊娠状态，表明TC可以影响平滑肌细胞的收缩活动的时间。
Telocytes (TCs) have been described in various organs and species (www.telocytes.com) as cells with telopodes (Tps) – very long cellular extensions with an alternation of thin segments (podomers) and dilated portions (podoms). We examined TCs using electron microscopy (EM), immunohistochemistry (IHC), immunofluorescence (IF), time-lapse videomicroscopy and whole-cell patch voltage clamp. EM showed a three-dimensional network of dichotomous-branching Tps, a labyrinthine system with homocellular and heterocellular junctions. Tps release extracellular vesicles (mean diameter of 160.6±6.9 nm in non-pregnant myometrium and 171.6±4.6 nm in pregnant myometrium), sending macromolecular signals to neighbouring cells. Comparative measurements (non-pregnant and pregnant myometrium) of podomer thickness revealed values of 81.94±1.77 vs 75.53±1.81 nm, while the podoms' diameters were 268.6±8.27 vs 316.38±17.56 nm. IHC as well as IF revealed double c-kit and CD34 positive results. Time-lapse videomicroscopy of cell culture showed dynamic interactions between Tps and myocytes. In non-pregnant myometrium, patch-clamp recordings of TCs revealed a hyperpolarisation-activated chloride inward current with calcium dependence and the absence of L-type calcium channels. TCs seem to have no excitable properties similar to the surrounding smooth muscle cells (SMCs). In conclusion, this study shows the presence of TCs as a distinct cell type in human non-pregnant and pregnant myometrium and describes morphometric differences between the two physiological states. In addition, we provide a preliminary in vitro electrophysiological evaluation of the non-pregnant state, suggesting that TCs could influence timing of the contractile activity of SMCs.