Determination of genes involved in the early process of molar root development initiation in rat by modified subtractive hybridization
Determination of genes involved in the early process of molar root development initiation in rat by modified subtractive hybridization
复制标题
通过改良消减杂交法测定参与大鼠磨牙根发育起始早期过程的基因。
DOI：
10.1016/j.bbrc.2007.09.087
复制
发表时间：
2007-11-30
影响因子：
3.1
通讯作者：
Jin, Yan
中科院分区：
文献类型：
--
作者：
Xing, Xianghui;Deng, Zhihong;Jin, Yan
大鼠磨牙和门牙有不同的根模式机制：前者形成多根模式，而后者形成单根模拟。但是控制根系模式的遗传信号却知之甚少。本研究首先观察到在胚胎第19天磨牙和切牙开始分化发育：磨牙形成双层根鞘细胞，而切牙形成颈环，以确定可能导致磨牙牙根发育的特殊分子信号。利用消减杂交技术成功构建了大鼠磨牙和切牙组织的消减cDNA文库。通过斑点杂交和测序对差异表达的基因克隆进行评价。发现Se 111、Nfic、Edar、GATA 6和一些新基因的差异表达，这些基因与牙根构型密切相关。预计对已鉴定基因的进一步研究将有助于了解它们在牙根形成中的具体作用。(C)2007年爱思唯尔公司All rights reserved.
Rat molars and incisors have different root patterning mechanisms: the former form a multirooted pattern while the latter form a single-rooted analogue. But the genetic signals that control root patterning are poorly understood. In this study, to identify the special molecular signals which may lead to the molar root development, we firstly observed that at embryonic day 19 the molar and the incisor began differentially developing: the molar formed double-layered cells of the root sheath while the incisor formed a cervical loop. By using the subtractive hybridization method, we successfully constructed a subtraction cDNA library of the rat molar and incisor tissues. Differentially expressed gene clones were evaluated by dot-blot and sequencing. Se111, Nfic, Edar, GATA6, and some novel genes were found differentially expressed, which were strongly related the tooth root patterning. It is anticipated that further study of genes identified will provide insights into their specific roles in the tooth root patterning. (C) 2007 Elsevier Inc. All rights reserved.