Bacillus subtilis KLBMPGC81 suppresses appressorium-mediated plant infection by altering the cell wall integrity signaling pathway and multiple cell biological processes in Magnaporthe oryzae.
Bacillus subtilis KLBMPGC81 suppresses appressorium-mediated plant infection by altering the cell wall integrity signaling pathway and multiple cell biological processes in Magnaporthe oryzae.
复制标题
枯草芽孢杆菌 KLBMPGC81 通过改变稻瘟病菌细胞壁完整性信号通路和多个细胞生物过程来抑制附着胞介导的植物感染
DOI：
10.3389/fcimb.2022.983757
复制
发表时间：
2022
影响因子：
5.7
通讯作者：
中科院分区：
文献类型：
--
作者：
稻瘟病菌是世界上最具破坏性的农作物病原菌之一，每年给水稻生产造成巨大损失。枯草芽孢杆菌是一种潜在的生物防治剂，已被开发用于许多作物系统，因为它是一种有效的生物活性化合物生产者。然而，这些药剂控制稻瘟病的机制还不完全清楚。我们证明了B。枯草杆菌KLBMPGC 81（KC 81）及其上清（KLBMPGC 81）对M. 11号菌株。为了更好地开发利用KC 81作为生防菌，研究了KC 81对稻瘟病病原菌的抑制机理。本研究表明，KC81微球对稻瘟病有较好的防治效果。结果表明，添加适量的柠檬酸盐对M.真菌和附着胞介导的植物感染。KC 81 β损害细胞壁完整性、微管和肌动蛋白细胞骨架、有丝分裂和自噬，所有这些都是M.菌丝生长、附着胞发育和寄主感染。我们进一步表明，通过增强Cdc2 Tyr 15的磷酸化，从而削弱M中的有丝分裂，从而降低细胞周期蛋白依赖性激酶Cdc2的活性。电池。细胞壁传感器MoWsc 1激活细胞壁完整性途径和Mps 1和Pmk 1丝裂原活化蛋白激酶。综上所述，我们的研究结果表明，KC 81是一种有效的杀菌剂，抑制M。通过异常激活细胞壁完整性途径，破坏细胞骨架，有丝分裂和自噬，促进细胞生长，附着胞形成和宿主感染。
Magnaporthe oryzae is one of the most destructive crop pathogens in the world, causing huge losses in rice harvest every year. Bacillus subtilis is a potential biocontrol agent that has been explored in many crop systems because it is a potent producer of bioactive compounds. However, the mechanisms by which these agents control rice blasts are not fully understood. We show that B. subtilis KLBMPGC81 (KC81) and its supernatant (SUP) have high antimicrobial activity against M. oryzae strain Guy11. To better exploit KC81 as a biocontrol agent, the mechanism by which KC81 suppresses rice blast pathogens was investigated. This study shows that KC81 SUP is effective in controlling rice blast disease. The SUP has a significant effect on suppressing the growth of M. oryzae and appressorium-mediated plant infection. KC81 SUP compromises cell wall integrity, microtubules and actin cytoskeleton, mitosis, and autophagy, all of which are required for M. oryzae growth, appressorium development, and host infection. We further show that the SUP reduces the activity of the cyclin-dependent kinase Cdc2 by enhancing the phosphorylation of Cdc2 Tyr 15, thereby impairing mitosis in M. oryzae cells. SUP induces the cell wall sensor MoWsc1 to activate the cell wall integrity pathway and Mps1 and Pmk1 mitogen-activated protein kinases. Taken together, our findings reveal that KC81 is an effective fungicide that suppresses M. oryzae growth, appressorium formation, and host infection by abnormally activating the cell wall integrity pathway, disrupting the cytoskeleton, mitosis, and autophagy.
登录
查看更多内容
影响因子：
6.7
作者：
Liu H;Zhang S;Ma J;Dai Y;Li C;Lyu X;Wang C;Xu JR
通讯作者：
Xu JR
影响因子：
16
作者：
Kroemer G;Mariño G;Levine B
通讯作者：
Levine B
影响因子：
2.6
作者：
Gualtieri, T;Ragni, E;Popolo, L
通讯作者：
Popolo, L
影响因子：
4.1
作者：
Fira, Djordje;Dimkic, Ivica;Stankovic, Slavisa
通讯作者：
Stankovic, Slavisa
影响因子：
3.5
作者：
Jeon, Junhyun;Goh, Jaeduk;Lee, Yong-Hwan
通讯作者：
Lee, Yong-Hwan