Tendon-derived stem cells undergo spontaneous tenogenic differentiation
Tendon-derived stem cells undergo spontaneous tenogenic differentiation
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DOI：
10.1016/j.yexcr.2016.01.007
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发表时间：
2016-02-01
影响因子：
3.7
通讯作者：
Li, Gang
中科院分区：
文献类型：
--
作者：
Guo, Jia;Chan, Kai-Ming;Li, Gang
肌腱衍生干细胞（TDSC）是完整肌腱组织中存在的干细胞亚群，具有自我更新、克隆形成和三谱系分化的能力。与骨髓来源的间充质干细胞（BMSC）相比，TDSC 在肌腱损伤修复方面更优越，因为它们保留了一些肌腱组织特异性的分化特性。在本研究中，发现TDSC会发生自发的肌腱分化，其中在TDSC培养（无肌腱诱导培养基）中，随着时间的推移，肌腱标记物的表达增加，而干性标记物的表达减少。在此过程中进一步合成胶原蛋白的能力也相应增强。经过较长时间的培养，单层TDSCs形成了具有丰富典型肌腱组织细胞外基质的“3D”层。此外，关键的肌腱转录因子Scx、Mkx、Egr1和Eya1在此过程中均上调。最后，我们将TDSCs的自发肌腱分化与TGF-β1诱导的TDSCs肌腱分化进行了比较，结果表明，TDSCs的自发肌腱分化是TDSCs的一般特征，与肌腱诱导下的TDSCs的效果相似但较弱。综上所述，本研究发现TDSC具有自发肌腱分化的潜力，这可能是治疗肌腱损伤的更好的细胞来源。 (C) 2016 Elsevier Inc. 保留所有权利。
Tendon-derived stem cell (TDSC) is a subpopulation of residing stem cells within the intact tendon tissues, with the capacities of self-renewal, clonogenicity, and three-lineage differentiation. Compared with bone marrow derived mesenchymal stem cells (BMSCs), TDSCs are superior for tendon injuries repair as they remain some tendon tissue-specific differentiation properties. In the present study, TDSC was found to undergo spontaneous tenogenic differentiation in which the expression of tenogenic markers were increased while the expression of stemness markers decreased with time in TDSCs culture (without tenogenic induction medium). The further collagen synthesis ability was correspondingly increased during this process. After a longer period of culture, the monolayer of TDSCs formed a "3D" layers with rich extracellular matrices of typical tendon tissues. In addition, the key tenogenic transcription factors, such as Scx, Mkx, Egr1 and Eya1 were all up-regulated in this process. Finally, we compared the spontaneous tenogenic differentiation with TGF-beta 1-induced tenogenic differentiation of TDSCs, and the results showed that the spontaneous tenogenic differentiation of TDSCs was general character of TDSCs, similar to but weaker than the effect of TDSCs under tenogenic induction. Taken together, the present study identified that TDSCs had the potential of spontaneous tenogenic differentiation, which may be a better cell source for the treatment of tendon injury. (C) 2016 Elsevier Inc. All rights reserved.