Magnetic beads-based electrochemiluminescence assay for rapid and sensitive detection of telomerase activity
Magnetic beads-based electrochemiluminescence assay for rapid and sensitive detection of telomerase activity
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DOI：
10.1016/j.elecom.2008.01.039
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发表时间：
2008-04
影响因子：
5.4
通讯作者：
Xiaoming Zhou;D. Xing;Debin Zhu;Li Jia
中科院分区：
文献类型：
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作者：
Xiaoming Zhou;D. Xing;Debin Zhu;Li Jia
端粒酶是一种潜在的癌症标志物。我们开发了一种新的、强大的端粒酶活性检测方法，它将改良的端粒重复扩增方案 (TRAP) 与基于磁珠的电化学发光 (ECL) 检测相结合。该测定的高性能与从单细胞水平测定端粒酶活性有关，并且 ECL 强度在 1-1000 HeLa 细胞当量范围内呈线性。所提出的端粒酶测定法为目前可用的端粒酶测定法提供了一种高度成本和时间有效的替代方案，而目前可用的端粒酶测定法受到繁琐且复杂的 PCR 后检测的限制。
Telomerase is a potential cancer marker. We developed a new and robust telomerase activity assay which combines the modified telomere repeat amplification protocol (TRAP) with magnetic beads-based electrochemiluminescence (ECL) detection. The high performance of this assay is related to the determination of telomerase activity from single cell levels, and ECL intensity is linear over the range of 1–1000 HeLa cell equivalents. The proposed telomerase assay offers a highly cost- and time-effective alternative to presently available telomerase assays, which are limited by tedious and complicated post-PCR detection.