Surface chemistries for antibody microarrays.
Surface chemistries for antibody microarrays.
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DOI：
10.2741/2362
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发表时间：
2007-05
期刊：
影响因子：
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通讯作者：
Shannon L. Seurynck-Servoss;Cheryl L. Baird;K. Rodland;R. Zangar
中科院分区：
文献类型：
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作者：
Shannon L. Seurynck-Servoss;Cheryl L. Baird;K. Rodland;R. Zangar
酶联免疫吸附测定（ELISA）微阵列有望成为检测疾病生物标志物的有力工具。最初用于打印ELISA微阵列芯片和在载玻片上捕获抗体的技术源自DNA微阵列领域。然而，由于需要在固定化时保持抗体结构和功能，用于DNA微阵列的表面化学并不总是适用于ELISA微阵列。为了确定更好的表面化学抗体捕获，一些商业公司和学术研究小组已经开发了新的载玻片类型，可以提高抗体在微阵列应用中的功能。在这篇综述中，我们比较和对比市售的载玻片化学，以及突出一些有前途的最新进展，在该领域。
Enzyme-linked immunosorbent assay (ELISA) microarrays promise to be a powerful tool for the detection of disease biomarkers. The original technology for printing ELISA microarray chips and capturing antibodies on slides was derived from the DNA microarray field. However, due to the need to maintain antibody structure and function when immobilized, surface chemistries used for DNA microarrays are not always appropriate for ELISA microarrays. In order to identify better surface chemistries for antibody capture, a number of commercial companies and academic research groups have developed new slide types that could improve antibody function in microarray applications. In this review we compare and contrast the commercially available slide chemistries, as well as highlight some promising recent advances in the field.