Classical swine fever virus NS5A regulates viral RNA replication through binding to NS5B and 3′UTR
Classical swine fever virus NS5A regulates viral RNA replication through binding to NS5B and 3′UTR
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DOI：
10.1016/j.virol.2012.04.014
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发表时间：
2012-10-25
期刊：
影响因子：
3.7
通讯作者：
Xiao, Ming
中科院分区：
文献类型：
--
作者：
Chen, Yan;Xiao, Jun;Xiao, Ming
在本报告中，猪瘟病毒（CSFV）NS5A当其浓度达到并超过NS5B的水平时，会抑制病毒RNA复制。 CSFV NS5A 的三个氨基酸片段（137-172、224-268 和 390-414）分别被证明对于 NS5B 结合至关重要。前两个片段对于病毒 RNA 复制的调节是独立必需的，并且与 NS5B 和 3'UTR 结合活性相关。我们还发现 CSFV NS5A 的氨基酸 W143、V145、P227、T246、P257、K399、T401、E406 和 L413 对于 NS5B 结合活性至关重要。此外，这些氨基酸被证明是病毒RNA复制和感染所必需的，并且在CSFV、BDV、BVDV和HCV的NS5A蛋白中保守。这些结果表明NS5A可能通过与NS5B和3'UTR结合来调节病毒RNA复制。当无法与 NS5B 结合时，NS5A 仍然可以通过与 3'UTR 结合来调节病毒 RNA 合成。 (C) 2012 年，爱思唯尔公司出版。
In this report, classical swine fever virus (CSFV) NS5A inhibit viral RNA replication when its concentration reached and surpassed the level of NS5B. Three amino acid fragments of CSFV NS5A, 137-172, 224-268 and 390-414 individually were shown to be essential to NS5B binding. The former two fragments were independently necessary for regulation of viral RNA replication and correlated with NS5B and 3'UTR binding activity. We also found that amino acids W143, V145, P227, T246, P257, K399, T401, E406 and L413 of CSFV NS5A were essential to NS5B binding activity. Furthermore, these amino acids were shown to be necessary for viral RNA replication and infection and conserved in NS5A proteins of CSFV, BDV, BVDV and HCV. These results indicated that NS5A may regulate viral RNA replication by binding to NS5B and 3'UTR. NS5A can still regulate viral RNA synthesis through binding to 3'UTR when binding to NS5B is not available. (C) 2012 Published by Elsevier Inc.