High-throughput behavioral screening method for detecting auditory response defects in zebrafish
High-throughput behavioral screening method for detecting auditory response defects in zebrafish
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DOI：
10.1016/s0165-0270(02)00118-8
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发表时间：
2002-08-30
影响因子：
3
通讯作者：
Sewell, WF
中科院分区：
文献类型：
--
作者：
Bang, PI;Yelick, PC;Sewell, WF
我们已经开发了一种自动化的，高通量的行为筛选方法，用于检测斑马鱼的听力缺陷。我们的分析监测了对响亮声音的快速逃避反射。用这种方法，36条成年斑马鱼，限制在视觉上隔离的车厢，可以同时评估近场400 Hz正弦纯音的反应。响应的自动化、客观确定是通过计算机程序实现的，该计算机程序在相对于声刺激的精确时间获得图像。在刺激呈现之前和之后用CCD摄像机拍摄的图像被减去以揭示对声音的响应。每小时最多可筛选108条鱼。超过6500条鱼进行了测试，以验证分析的可靠性。我们发现这些动物中有1%表现出听力障碍。通过放射学分析进一步评估无应答者的表型，以确定听觉系统的总体形态缺陷。几乎所有这些都显示出听觉系统传导元件的异常：鱼鳔或韦伯小骨。(C)2002 Elsevier Science B.V.保留所有权利。
We have developed an automated, high-throughput behavioral screening method for detecting hearing defects in zebrafish. Our assay monitors a rapid escape reflex in response to a loud sound. With this approach, 36 adult zebrafish, restrained in visually isolated compartments, can be simultaneously assessed for responsiveness to near-field 400 Hz sinusoidal tone bursts. Automated, objective determinations of responses are achieved with a computer program that obtains images at precise times relative to the acoustic stimulus. Images taken with a CCD video camera before and after stimulus presentation are subtracted to reveal a response to the sound. Up to 108 fish can be screened per hour. Over 6500 fish were tested to validate the reliability of the assay. We found that 1% of these animals displayed hearing deficits. The phenotypes of non-responders were further assessed with radiological analysis for defects in the gross morphology of the auditory system. Nearly all of those showed abnormalities in conductive elements of the auditory system: the swim bladder or Weberian ossicles. (C) 2002 Elsevier Science B.V. All rights reserved.