Basonuclin: a novel mammalian maternal-effect gene
Basonuclin: a novel mammalian maternal-effect gene
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DOI：
10.1242/dev.02371
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发表时间：
2006-05-15
期刊：
影响因子：
4.6
通讯作者：
Tseng, H
中科院分区：
文献类型：
--
作者：
Ma, J;Zeng, FY;Tseng, H
碱性核蛋白是一种锌指蛋白，在卵母细胞中大量存在。由于着床前胚胎核素的来源是母体，因此它有资格成为母体效应基因。使用转基因rnai方法，我们在小鼠卵母细胞中特异性地敲低了导致雌性低生育能力的basonuclin。卵母细胞中碱性核素缺乏扰乱了RNA聚合酶I和RNA聚合酶ii介导的转录，卵母细胞形态受到影响（细胞质和细胞表面异常证明）。然而，一些受影响的卵母细胞仍然可以成熟并在中期II停止，并排卵。然而，受精卵缺乏碱性核蛋白未能发育超过两细胞阶段，这种着床前的失败解释了亚生育表型。这些结果表明，碱性核蛋白是哺乳动物母性效应基因的新成员，有趣的是，它与先前报道的哺乳动物母性效应基因不同，它也明显干扰卵子发生。
Basonuclin is a zinc-finger protein found in abundance in oocytes. It qualifies as a maternal-effect gene because the source of preimplantation embryonic basonuclin is maternal. Using a transgenic-RNAi approach, we knocked down basonuclin specifically in mouse oocytes, which led to female sub-fertility. Basonuclin deficiency in oocytes perturbed both RNA polymerase I- and II-mediated transcription, and oocyte morphology was affected ( as evidenced by cytoplasmic and cell surface abnormalities). Some of the affected oocytes, however, could still mature to and arrest at metaphase II, and be ovulated. Nevertheless, fertilized basonuclin-deficient eggs failed to develop beyond the two-cell stage, and this pre-implantation failure accounted for the sub-fertility phenotype. These results suggest that basonuclin is a new member of the mammalian maternal-effect genes and, interestingly, differs from the previously reported mammalian maternal-effect genes in that it also apparently perturbs oogenesis.