Retention of multilineage differentiation potential of mesenchymal cells during proliferation in response to FGF
Retention of multilineage differentiation potential of mesenchymal cells during proliferation in response to FGF
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DOI：
10.1006/bbrc.2001.5777
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发表时间：
2001-10-26
影响因子：
3.1
通讯作者：
Kato, Y
中科院分区：
文献类型：
--
作者：
Tsutsumi, S;Shimazu, A;Kato, Y
骨髓间充质干细胞（MSC）可以分化为各种结缔组织细胞，如果MSC可以在体外扩增，则可用于骨、软骨和肌腱缺损的自体细胞移植。然而，MSC的寿命短，其在培养中的分化潜力降低，限制了其临床应用。本研究的目的是鉴定一种参与MSC自我更新和维持其多向分化潜能的生长因子。成纤维细胞生长因子-2（FGF-2）显著增加兔、犬和人骨髓MSC单层培养物的生长速率和寿命。FGF-2的这种作用在低密度培养物中比在高密度培养物中更突出。此外，所有的MSC在体外扩增与FGF-2，但不是没有FGF-2，分化成软骨细胞在小球培养。FGF（+）MSC在许多有丝分裂中也保留了成骨和成脂潜能。这些发现表明FGF在MSC的自我更新中起着至关重要的作用。(C)北京：科学出版社.
Mesenchymal stem cells (MSC) that can differentiate to various connective tissue cells may be useful for autologous cell transplantation to defects of bone, cartilage, and tendon, if MSC can be expanded in vitro. However, a short life span of MSC and a reduction in their differentiation potential in culture have limited their clinical application. The purpose of this study is to identify a growth factor(s) involved in self-renewal of MSC and the maintenance of their multilineage differentiation potential. Fibroblast growth factor-2 (FGF-2) markedly increased the growth rate and the life span of rabbit, canine, and human bone marrow MSC in monolayer cultures. This effect of FGF-2 was more prominent in low-density cultures than in high-density cultures. In addition, all MSC expanded in vitro with FGF-2, but not without FGF-2, differentiated to chondrocytes in pellet cultures. The FGF(+) MSC also retained the osteogenic and adipogenic potential throughout many mitotic divisions. These findings suggest that FGFs play a crucial role in self-renewal of MSC. (C) 2001 Academic Press.