Gibberellins promote flowering of Arabidopsis by activating the LEAFY promoter
Gibberellins promote flowering of Arabidopsis by activating the LEAFY promoter
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DOI：
10.1105/tpc.10.5.791
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发表时间：
1998-05-01
期刊：
影响因子：
11.6
通讯作者：
Weigel, D
中科院分区：
文献类型：
--
作者：
Blázquez, MA;Green, R;Weigel, D
植物激素的赤霉素类已涉及在几个物种的开花控制。在拟南芥中，内源赤霉素的严重减少会延迟长日照开花，并阻止短日照开花。我们研究了赤霉素对开花的不同影响与LEAR基因表达的关系。我们已经发现赤霉素缺陷的ga 1 -3突变体在短日照内不能开花是由于缺乏LEAN启动子诱导所致。组成型表达的LEAN转基因在短时间内恢复ga 1 -3突变体开花的能力证实了这两个事件之间的因果关系。与短日照相反，赤霉素生物合成的损伤仅导致LEAN表达的减少，当植物生长在长日照或蔗糖在黑暗中。作为鉴定其他可能调节开花的小分子的第一步，我们已经开发了一种快速的LEAFY启动子活性体外测定法。
The gibberellin class of plant hormones has been implicated in the control of flowering in several species. In Arabidopsis, severe reduction of endogenous gibberellins delays flowering in long days and prevents flowering in short days. We have investigated how the differential effects of gibberellins on flowering correlate with expression of LEAR: a floral meristem identity gene. We have found that the failure of gibberellin-deficient ga1-3 mutants to flower in short days was paralleled by the absence of LEAN promoter induction. A causal connection between these two events was confirmed by the ability of a constitutively expressed LEAN transgene to restore flowering to ga1-3 mutants in short days. In contrast to short days, impairment of gibberellin biosynthesis caused merely a reduction of LEAN expression when plants were grown in long days or with sucrose in the dark. As a first step toward identifying other small molecules that might regulate flowering, we have developed a rapid in vitro assay for LEAFY promoter activity.