Soil acid and alkaline phosphatase activity as pH adjustment indicators
Soil acid and alkaline phosphatase activity as pH adjustment indicators
复制标题
DOI：
10.1016/s0038-0717(00)00166-8
复制
发表时间：
2000-11-01
影响因子：
9.7
通讯作者：
Wang, P
中科院分区：
文献类型：
--
作者：
Dick, WA;Cheng, L;Wang, P
土壤肥力和作物生产受到生物过程的影响，而这些过程，包括酶活性，受pH值的影响。我们研究了碱性磷酸酶（AlkP）和酸性磷酸酶（AcdP）活性的潜力，以确定作物生产的最佳土壤pH值和实现这一最佳所需的石灰量。5种不同性质的酸性土壤分别以土壤需要量的0,0.2,0.5,1.0和2.0倍的CaCO3处理。为了消除土壤对酶的绝对活性值的影响，采用了AlkP/AcdP活性比来测试土壤的反应。alp /AcdP的比值对CaCO3添加引起的pH变化有直接响应，约0.5的alp /AcdP比值将土壤分为pH适当调整的土壤和仍需额外石灰处理的土壤。然而，在石灰处理的土壤培养67天后，用有机改良剂（包括细磨鸡粪和苜蓿渣）处理土壤，将AlkP/AcdP比提高到约3.0。对于严重依赖自然生物过程来维持生产力的种植系统，测量碱性磷/AcdP比值可能比化学方法更适合评估有效土壤pH值和石灰需求。(C) 2000 Elsevier Science Ltd.版权所有。
Soil fertility and crop production are affected by biological processes and these processes, including enzyme activites, are influenced by pH. We investigated the potential of using alkaline phosphatase (AlkP) and acid phosphatase (AcdP) activities, for determining the optimum soil pH for crop production and the amount of lime required to achieve this optimum. Five acid soils, which varied widely in selected properties, were treated with CaCO3 at rates of 0, 0.2, 0.5, 1.0 and 2.0 x the soil's lime requirement needs. To remove soil variations in absolute enzyme activity values, an AlkP/AcdP activity ratio was used to test soil response. The ratios of AlkP/AcdP responded immediately to the changes in pH caused by CaCO3 additions and an AlkP/AcdP ratio of approximately 0.5 divided soils into those with appropriate pH adjustment and those still needing additional lime treatment. However, incubation of the lime-treated soils for 67 days followed by treating the soils with organic amendments (which included finely ground chicken manure and alfalfa residues) increased the AlkP/AcdP ratios to approximately 3.0. For cropping systems that rely heavily on natural biological processes to maintain productivity, measuring the AlkP/AcdP ratio may be preferable to chemical approaches for evaluating effective soil pH and liming needs. (C) 2000 Elsevier Science Ltd. All rights reserved.