A Symbiotic Virus Facilitates Aphid Adaptation to Host Plants by Suppressing Jasmonic Acid Responses
A Symbiotic Virus Facilitates Aphid Adaptation to Host Plants by Suppressing Jasmonic Acid Responses
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共生病毒通过抑制茉莉酸反应促进蚜虫适应宿主植物
DOI：
10.1094/mpmi-01-19-0016-r
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发表时间：
2020-01-01
影响因子：
3.5
通讯作者：
Cui, Feng
中科院分区：
文献类型：
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作者：
Lu, Hong;Zhu, Junjie;Cui, Feng
共生病毒存在于许多昆虫中，然而，它们在宿主昆虫中的功能尚不清楚。本研究探讨了豌豆蚜病毒（Acyrthosiphonpisumvirus，APV）在豌豆蚜与植物互作中的作用。APV主要定位于蚜虫的唾液腺和肠道中，并在昆虫体内繁殖。APV在蚜虫取食过程中水平传播到寄主植物，但病毒不在寄主植物中复制。当蚜虫的豌豆寄主品种定殖两种低适合度植物时，蚜虫和植物中的病毒滴度都显著增加。此外，APV的感染强烈促进豌豆寄主小种在V. villosa上的存活率。转录组学分析表明，只有0.85%的蚜虫基因对APV感染有反应，当蚜虫取食V. villosa时，转录水平的变化不超过4倍。由于APV感染的存活率的提高显然与病毒对植物激素茉莉酸（JA）和JA-异亮氨酸水平的抑制作用有关。我们的数据表明共生病毒对其蚜虫宿主的益处，并展示了共生病毒介导的三种相互作用的新情况。
Symbiotic viruses exist in many insects; however, their functions in host insects are not well understood. In this study, we explored the role of acyrthosiphon pisum virus (APV) in the interaction of its host aphid Acyrthosiphon pisum with plants. APV is primarily located in aphid salivary glands and gut and propagated in the insect. APV is horizontally transmitted to host plants during aphid feeding, but the virus does not replicate in the host plant. When the pea host race of aphids colonized two low-fitness plants, Medicago truncatula and Vicia villosa, the virus titers in both the aphids and plants significantly increased. Furthermore, APV infection strongly promoted the survival rate of the pea host race on V. villosa. Transcriptomic analysis showed that only 0.85% of aphid genes responded to APV infection when aphids fed on V. villosa, with a fold change in transcript levels of no more than fourfold. The improved survival due to APV infection was apparently related to the inhibitory effect of the virus on levels of phytohormone jasmonic acid (JA) and JA-isoleucine. Our data suggest a benefit of the symbiotic virus to its aphid host and demonstrate a novel case of symbiotic virus-mediated three-species interaction.