ICP-MS analysis of lanthanide-doped nanoparticles as a non-radiative, multiplex approach to quantify biodistribution and blood clearance.
ICP-MS analysis of lanthanide-doped nanoparticles as a non-radiative, multiplex approach to quantify biodistribution and blood clearance.
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DOI：
10.1016/j.biomaterials.2011.10.077
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发表时间：
2012-02
期刊：
影响因子：
14
通讯作者：
Tsourkas, Andrew
中科院分区：
文献类型：
--
作者：
Crayton, Samuel H.;Elias, Drew R.;Al Zaki, Ajlan;Cheng, Zhiliang;Tsourkas, Andrew
材料科学和化学的最新进展已经导致具有不同物理化学性质（例如尺寸、电荷、形状和表面化学）的纳米颗粒的发展。评价哪种物理化学性质最适合成像和治疗研究是具有挑战性的，这不仅是因为要评价的样品数量众多，而且还因为与体内研究相关的实验变异性很大（例如肿瘤大小、注射剂量、受试者体重等的差异）。为了解决这个问题，我们开发了一种镧系元素掺杂的纳米颗粒系统和分析方法，可以同时定量比较多种纳米颗粒成分。具体而言，合成了具有一系列不同尺寸和电荷的超顺磁性氧化铁（SPIO），每种都具有独特的镧系元素掺杂剂。在将各种SPIO组合物同时注射到荷瘤小鼠中后，使用电感耦合等离子体质谱法（ICP-MS）定量和正交地评估连续血液样品和切除的肿瘤和器官中每种SPIO组合物的浓度。该方法被证明可推广到其他纳米颗粒平台，包括树枝状聚合物，脂质体和聚合物囊泡。该方法提供了一种简单、成本有效且非辐射的方法，以同时定量比较超过10种纳米颗粒组合物的肿瘤定位、生物分布和血液清除率，消除了受试者之间的差异性。
Recent advances in material science and chemistry have led to the development of nanoparticles with diverse physicochemical properties, e.g. size, charge, shape, and surface chemistry. Evaluating which physicochemical properties are best for imaging and therapeutic studies is challenging not only because of the multitude of samples to evaluate, but also because of the large experimental variability associated with in vivo studies (e.g. differences in tumor size, injected dose, subject weight, etc.). To address this issue, we have developed a lanthanide-doped nanoparticle system and analytical method that allows for the quantitative comparison of multiple nanoparticle compositions simultaneously. Specifically, superparamagnetic iron oxide (SPIO) with a range of different sizes and charges were synthesized, each with a unique lanthanide dopant. Following the simultaneous injection of the various SPIO compositions into tumor-bearing mice, inductively coupled plasma mass spectroscopy (ICP-MS) was used to quantitatively and orthogonally assess the concentration of each SPIO composition in serial blood samples and the resected tumor and organs. The method proved generalizable to other nanoparticle platforms, including dendrimers, liposomes, and polymersomes. This approach provides a simple, cost-effective, and non-radiative method to quantitatively compare tumor localization, biodistribution, and blood clearance of more than 10 nanoparticle compositions simultaneously, removing subject-to-subject variability.
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