VIP innervation: sharp contrast in fetal sheep and baboon adrenal glands suggests differences in developmental regulation.
VIP innervation: sharp contrast in fetal sheep and baboon adrenal glands suggests differences in developmental regulation.
复制标题
VIP 神经支配：胎羊和狒狒肾上腺的鲜明对比表明发育调节的差异。
DOI：
10.1016/s0006-8993(00)02683-4
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发表时间：
2000
期刊：
影响因子：
2.9
通讯作者：
McDonald,TJ
中科院分区：
文献类型：
--
作者：
Berghorn,KA;Li,C;Nathanielsz,PW;McDonald,TJ
应用免疫细胞化学技术和光镜技术，对妊娠85%的绵羊胎羊肾上腺皮质和髓质中血管活性肠肽(VIP)与酪氨酸羟基酶的关系进行了研究。VIP免疫组织化学染色在胎羊肾上腺皮质神经纤维和细胞中呈强阳性，而在狒狒的细胞中VIP免疫染色较弱，不存在。此外，酪氨酸羟基酶免疫阳性细胞存在于胎羊和狒狒的肾上腺皮质中，在胎羊中被VIP免疫反应神经纤维大量支配，而在胚胎狒狒中则不表达。胚胎绵羊肾上腺皮质血管活性肠肽免疫阳性纤维的出现频率(14.82±3.10vs.0.84±0.26纤维/野)、直径(2.93±0.34vs.0.93±0.07μm)和横切面距离(127.85±5.16vs.74.53±4.93μm)均大于胎羊(P<0.05)。绵羊胎儿肾上腺髓质细胞(神经节和嗜铬细胞)和纤维具有较强的VIP免疫原性，而狒狒则不具免疫原性。胎儿绵羊延髓的血管活性肠肽纤维直径小于胎羊皮质(1.2 2±0.13比2.93±0.34μm，P<0.0 5)，但与外源性神经纤维(1.3 0±0.0 9μm)相比无显著差异。我们推测，在这个年龄段的胎羊中，延髓神经纤维主要来自外部来源，而皮质纤维来自皮质神经节细胞。我们的结论是，在妊娠85%时，胎儿绵羊的肾上腺皮质和髓质中的VIP神经控制旁分泌(如糖皮质激素/儿茶酚胺)相互作用的可能性比胎儿狒狒大得多。
Immunocytochemical technique and light microscopy were used to ascertain the relationship between vasoactive intestinal polypeptide (VIP) and tyrosine hydroxylase in fetal sheep and fetal baboon adrenal cortices and medullae at 85% of gestation. VIP immunostaining was extremely robust in fetal sheep adrenal cortical neurofibers and cells while weak in fibers and nonexistent in cells of fetal baboon. Also, tyrosine hydroxylase-immunopositive cells, present throughout the adrenal cortices of both fetal sheep and baboons, were heavily innervated by VIP-immunoreactive neurofibers in fetal sheep, but not in fetal baboons. Adrenal cortical VIP-immunopositive fibers occurred in greater (P<0.05) frequency in fetal sheep than in fetal baboons (14.82±3.10 vs. 0.84±0.26 fibers/field), were larger in diameter (2.93±0.34 vs. 0.93±0.07 μm) and ran for longer distances in the plane of section (127.85±5.16 vs. 74.53±4.93 μm). VIP immunogenicity in cells (ganglion and chromaffin) and fibers was robust in fetal adrenal medulla of sheep while nonexistent in baboons. VIP fibers in fetal sheep medulla were smaller in diameter compared to fetal sheep cortex (1.22±0.13 vs. 2.93±0.34 μm, P<0.05), but not compared to extrinsic nerve fibers (1.30±0.09 μm). We hypothesize that in fetal sheep of this age, medullary neurofibers derive primarily from extrinsic sources while cortical fibers arise from cortical ganglion cells. We conclude that at 85% of gestation the potential for VIP neural control of paracrine (e.g., glucocorticoid/catecholamine) interactions in both adrenal cortex and medulla is much greater in fetal sheep compared to fetal baboons.