The tissue distribution of the human β3-adrenoceptor studied using a monoclonal antibody:: Direct evidence of the β3-adrenoceptor in human adipose tissue, atrium and skeletal muscle
The tissue distribution of the human β3-adrenoceptor studied using a monoclonal antibody:: Direct evidence of the β3-adrenoceptor in human adipose tissue, atrium and skeletal muscle
复制标题
DOI：
10.1038/sj.ijo.0801039
复制
发表时间：
1999-10-01
影响因子：
4.9
通讯作者：
Murphy, GJ
中科院分区：
文献类型：
--
作者：
Chamberlain, PD;Jennings, KH;Murphy, GJ
目的：为了研制一种能识别人细胞和组织细胞外表面表达的天然β（3）-肾上腺素受体表位的单克隆抗体，设计：针对转染的哺乳动物细胞系表达的人β（3）-肾上腺素受体，制备了一种高亲和力的单克隆抗体Mab 72 c。在CHO在用β（3）-肾上腺素受体cDNA转染的（中国仓鼠卵巢）细胞中，发现抗体标记与通过放射性标记的β（3）-肾上腺素受体拮抗剂的结合测量的受体密度成比例，[I-125]碘氰基吲哚酚。Mab 72 c的使用已经证明了β（3）-肾上腺素受体在多种人体组织中的表达，包括胆囊、前列腺和结肠，在这些组织中先前已经检测到mRNA信号。这项研究还提供了第一个直接证明β（3）-肾上腺素能受体在人体骨骼肌，心房和脂肪tissue.CONCLUSION的表达：这种抗体的发展代表了一个重要的除了armentarium的试剂，可用于研究β（3）-肾上腺素能受体在人体组织中的定位。
OBJECTIVE: To develop a monoclonal antibody that recognises an epitope of the native beta(3)-adrenoceptor expressed on the extracellular surface of human cells and tissues.DESIGN: A high affinity monoclonal antibody, Mab72c, was raised against the human beta(3)-adrenoceptor expressed on a transfected mammalian cell line.RESULTS: In CHO (Chinese hamster ovary) cells transfected with beta(3)-adrenoceptor cDNA, antibody labelling was found to be proportional to receptor density measured by the binding of the radiolabelled beta(3)-adrenoceptor antagonist, [I-125]iodocyanopindolol. The use of Mab 72c has demonstrated the expression of the beta(3)-adrenoceptor in a variety of human tissues, including gall bladder, prostate and colon, where a mRNA signal had been detected previously. This study also provides the first direct demonstration of the expression of beta(3)-adrenoceptors in human skeletal muscle, atrium and adipose tissue.CONCLUSION: The development of this antibody represents an important addition to the armentarium of reagents that are available to study the localisation of beta(3)-adrenoceptors in human tissues.