Expressional characterization of two class I trehalose-6-phosphatesynthase genes in Hevea brasiliensis (para rubber tree) suggests a role in rubber production
Expressional characterization of two class I trehalose-6-phosphatesynthase genes in Hevea brasiliensis (para rubber tree) suggests a role in rubber production
复制标题
巴西橡胶树 (Hevea brasiliensis) 中两个 I 类海藻糖-6-磷酸合酶基因的表达特征表明其在橡胶生产中的作用
DOI：
10.1007/s11056-017-9578-4
复制
发表时间：
2017
期刊：
影响因子：
2.2
通讯作者：
Chaorong tang
中科院分区：
文献类型：
--
作者：
Binhui Zhou;Yongjun Fang;Yujie Fan;Ying Wang;Jiyan Qi;Chaorong tang
在植物中，Tre6P合成酶（Tre6P synthase， TPS）合成的海藻糖-6-磷酸（Tre6P）是一种信号代谢物，在植物生长发育过程中调控蔗糖代谢。heveagenome共预测了14个tps基因，其中两类itpmember hbtps1和2在各种激素和非生物胁迫处理下的表达。乙烯利（2-氯乙基膦酸）、2,4-二氯苯氧乙酸、茉莉酸和水杨酸处理对产胶乳汁管细胞质乳胶中hbtps1和hbtps2的表达有显著影响，但影响方式不同。hbtps1和hbtps2的表达在低温（5°C）和高温（40°C）条件下也以类似的方式显著上调。相比之下，干旱处理（20% PEG）对幼苗hbtps1和2的表达有不同的影响。例如，在根中，HbTPS1的转录本随着处理而积累，而hbtps2的转录本在前24小时内减少。在HbTPS1和hbtps2启动子中可以预测各种激素和应激响应元件，并且在转基因拟南芥的所有生长阶段都观察到HbTPS1::GUS活性的普遍表达。这些结果，再加上橡胶生物合成是一种防御反应，以蔗糖为前体分子，提出tps基因在调节橡胶生产中的潜在作用。
In plants, trehalose-6-phosphate (Tre6P) synthesized by the Tre6P synthase (TPS) acts as a signal metabolite in regulating sucrose metabolism in relation to plant growth and development. TheHeveagenome predicts a total of 14TPSgenes, and two class ITPSmembers,HbTPS1and2, were explored here for their expressions under various hormone and abiotic stress treatments.HbTPS1and2expressions in latex, cytoplasm of rubber-producing laticifers, were markedly affected by the treatments of ethephon (2-chloroethylphosphonic acid, an ethylene releaser), 2,4-dichlorophenoxyacetic acid, jasmonic acid and salicylic acid although in a distinct manner.HbTPS1and2expressions were significantly up-regulated in a similar manner by cold (5 °C) and heat (40 °C) as examined inHeveaseedling leaves and roots, and by wounding in latex. In comparison, the drought treatment (20% PEG) posed a different effect onHbTPS1and2expressions inHeveaseedlings. For example, in roots,HbTPS1transcripts accumulated with the treatment, whereas those ofHbTPS2decreased for the first 24 h. Various hormone- and stress-responsive elements could bein silicopredicted inHbTPS1and2promoters, and a universal expression was observed for the HbTPS1::GUS activity in all growth stages of transgenicArabidopsis. These results, coupled with the fact of rubber biosynthesis as a defense response using sucrose as the precursor molecule, propose a potential role ofTPSgenes in regulating rubber production.