Resonant electron tunneling through azurin in air and liquid by scanning tunneling microscopy
Resonant electron tunneling through azurin in air and liquid by scanning tunneling microscopy
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DOI：
10.1109/tnano.2005.851448
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发表时间：
2005-09-01
影响因子：
2.4
通讯作者：
Rinaldi, R
中科院分区：
文献类型：
--
作者：
Frascerra, V;Calabi, F;Rinaldi, R
利用扫描隧道显微镜（STM）首次研究了氧化还原金属蛋白azurin在缓冲溶液和空气中的电子转移。STM分析在这两种情况下提供了分子稳定和强大结合的证据。与蓝蛋白分子相关的亮点在STM图像中清晰可见。吸附蓝蛋白的图像对比度受偏压电位的影响很大，只有在某些明确的电压值下才能看到蛋白质。该实验证明了在空气中诱导蓝蛋白隧穿的可能性，为在非液体环境中工作的基于蛋白质的混合纳米器件的开发提供了非常有趣的前景。
Electron transfer through the redox metalloprotein azurin immobilized on An (111) by its disulphide bridge is studied by scanning tunneling microscopy (STM) in buffer solution and (for the first time) in air. STM analysis gives evidences of a stable and robust binding of the molecules in both cases. Bright spots, associated with azurin molecules, are clearly visible in STM images. The image contrast of adsorbed azurin is highly affected by the bias potential with proteins visible only for some well-defined voltage values. This experimental demonstration of the possibility to induce tunneling through azurin in air could disclose very interesting perspectives for the development of protein-based hybrid nanodevices operating in nonliquid environments.