Three division-competent, structurally-distinct cell populations contribute to murine mammary epithelial renewal
Three division-competent, structurally-distinct cell populations contribute to murine mammary epithelial renewal
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DOI：
10.1016/s0040-8166(97)80024-9
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发表时间：
1997-04-01
期刊：
影响因子：
2.6
通讯作者：
Smith, GH
中科院分区：
文献类型：
--
作者：
Chepko, G;Smith, GH
乳腺上皮细胞中分裂能力细胞的标志物对于理解正常和肿瘤性乳腺上皮细胞的生长和结构的连续性是重要的，但是很难揭示。使用浓缩染色体的存在作为分裂能力的指示，我们描述了细胞核和细胞质中的形态标志物，其可靠地表征两组结构不同的细胞，小鼠（即小鼠和大鼠）乳腺上皮中的分裂能力细胞。部分基于细胞大小、细胞核和细胞质染色特征以及细胞核和细胞质的独特形态学特征的标记物允许将有分裂能力的细胞分为两类：“大亮细胞”和“小亮细胞”，根据细胞质细胞器分化程度，小亮细胞是上皮中分化最少的细胞，大的轻细胞在结构和功能上似乎处于分泌分化的早期阶段。我们证明，使用细胞计数的统计分析，（每单位面积的上皮），事实上，在大鼠乳腺上皮中存在三种分裂能力细胞群，并且大的光细胞是终末分化细胞（分泌细胞和肌上皮细胞）的直接前体，利用我们的研究结果，我们合成了一个细胞有丝分裂的形态学模型和上皮细胞分化的进展，在小鼠乳腺从干细胞通过两个祖细胞。
Markers for the division-competent cells in mammary gland epithelium are important to the understanding of normal and neoplastic mammary epithelial growth and architectural maintainence, but have been difficult to reveal, Using the presence of condensed chromosomes as an indicator of division competence, we have described morphological markers in the nucleus and cytoplasm that reliably characterize two sets of structurally-distinct, division-competent cells in murine (i.e. mouse and rat) mammary epithelium. The markers, based, in part, on cell size, nuclear and cytoplasmic staining characteristics, and distinctive morphological features of the nucleus and cytoplasm allow classification of the division-competent cells into two categories: 'large light cells' and 'small light cells', Based on the degree of cytoplasmic organelle differentiation, the small light cells are the least differentiated cells in the epithelium, and the large light cells appear, structurally and functionally, to be in the early stages of secretory differentiation. We demonstrate, using statistical analysis of cell counts (per unit area of epithelium) from four stages of mammary epithelial growth, that there are, in fact, three division-competent cell populations in the rat mammary epithelium, and that the large light cell is a direct precursor to terminally differentiated cells, both secretory and myoepithelial, Using our results we synthesize a morphological model of cell mitosis and the progression of epithelial differentiation in murine mammary gland from a stem cell through two progenitors.