VISUAL RECEPTOR CYCLE IN NORMAL AND PERIOD MUTANT DROSOPHILA - MICROSPECTROPHOTOMETRY, ELECTROPHYSIOLOGY, AND ULTRASTRUCTURAL MORPHOMETRY
VISUAL RECEPTOR CYCLE IN NORMAL AND PERIOD MUTANT DROSOPHILA - MICROSPECTROPHOTOMETRY, ELECTROPHYSIOLOGY, AND ULTRASTRUCTURAL MORPHOMETRY
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DOI：
10.1017/s0952523800009585
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发表时间：
1992-08-01
影响因子：
1.9
通讯作者：
STARK, WS
中科院分区：
文献类型：
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作者：
CHEN, DM;CHRISTIANSON, JS;STARK, WS
在果蝇的12小时光照/12小时黑暗周期中测量视觉色素、灵敏度和横纹肌大小。在随后的恒定黑暗中测量视觉色素和灵敏度[暗/暗（D/D）]。MSP（显微分光光度法）和ERG（视网膜电图）分别显示了视觉色素和灵敏度的循环。光照后4小时，白眼（野生型）果蝇的视色素下降40%，2-4小时后恢复。在光照后4小时，ERG敏感性[在光照/暗（L/D）下的w / +果蝇中]下降了75%，超过了单独由视觉色素（MSP）变化介导的预期。ERG敏感度在光照前8小时开始下降，视色素在光照后2小时开始下降。这些循环在持续的黑暗中继续（D/D），表明存在昼夜节律。白眼期突变体的视色素水平和L/D敏感性周期相似；如果在D/D周期上有任何变化，那么per的变化是微妙的。采用电子显微形态测量法测定横纹肌横截面积。在L/D周期中，面积变化不大。
Visual pigment, sensitivity, and rhabdomere size were measured throughout a 12-h light/12-h dark cycle in Drosophila. Visual pigment and sensitivity were measured during subsequent constant darkness [dark/dark (D/D)]. MSP (microspectrophotometry) and the ERG (electroretinogram) revealed a cycling of visual pigment and sensitivity, respectively. A visual pigment decrease of 40% was noted at 4 h after light onset that recovered 2-4 h later in white-eyed (otherwise wild-type, w per+) flies. The ERG sensitivity [in w per+ flies in light/dark (L/D)] decreased by 75% at 4 h after light onset, more than expected if mediated by visual pigment (MSP) changes alone. ERG sensitivity begins decreasing 8 h before light onset while decreases in visual pigment begin 2 h after light onset. These cycles continue in constant darkness (D/D), suggesting a circadian rhythm. White-eyed period (per) mutants show similar cycles of visual pigment level and sensitivity in L/D; per's alterations, if any on the D/D cycles were subtle. The cross-sectional areas of rhabdomeres in w per+ were measured using electron micrographic (EM) morphometry. Area changed little through the L/D cycle.