HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 REPLICATION IN PERIPHERAL-BLOOD MONONUCLEAR-CELLS IN THE PRESENCE OF COCAINE
HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 REPLICATION IN PERIPHERAL-BLOOD MONONUCLEAR-CELLS IN THE PRESENCE OF COCAINE
复制标题
DOI：
10.1093/infdis/168.5.1157
复制
发表时间：
1993-11-01
影响因子：
6.4
通讯作者：
POMERANTZ, RJ
中科院分区：
文献类型：
--
作者：
BAGASRA, O;POMERANTZ, RJ
先前的研究表明，食用可卡因会改变动物的正常免疫功能。多项流行病学研究表明，可卡因消费与艾滋病风险增加之间存在关联。在本研究中，从 8 名健康的人类免疫缺陷病毒 (HIV) 1 型血清阴性志愿者中分离出未刺激的外周血单核细胞 (PBMC)，在体外接触可卡因或其副产品之一，其浓度与可卡因临床滥用期间发现的血液浓度相一致。与未处理的 PBMC 相比，在体外感染后，用可卡因处理的 PBMC 的 HIV-1 复制水平显着增加。可卡因的主要副产物苯甲酰爱康宁似乎对 HIV-1 复制没有任何显着的增强作用。可卡因或其副产品不会显着增加持续感染 HIV-1 的 T 淋巴细胞或单核细胞系中的 HIV-1 复制。这些数据表明，PBMC（而非长期感染 HIV-1 的细胞系）接触可卡因可导致 HIV-1 复制增加。
Previous studies have shown that cocaine consumption alters the normal immune functions of animals. Several epidemiologic studies have indicated an association between cocaine consumption and an increased risk for AIDS. In the present studies, unstimulated peripheral blood mononuclear cells (PBMC) isolated from 8 healthy, human immunodeficiency virus (HIV) type 1-seronegative volunteers were exposed to cocaine or one of its by-products, in vitro, at concentrations compatible with blood levels found during clinical abuse of cocaine. PBMC treated with cocaine had significantly increased levels of HIV-1 replication after infection in vitro compared with untreated PBMC. The major cocaine by-product, benzoylecgonine, did not appear to exert any significant potentiating effect on HIV-1 replication. Cocaine or its by-product did not significantly increase HIV-1 replication in persistently HIV-1-infected T lymphocytic or monocytoid cell lines. These data indicate that exposure of PBMC but not chronically HIV-1-infected cell lines to cocaine can result in increased HIV-1 replication.