The Xenopus platelet-derived growth factor alpha receptor: cDNA cloning and demonstration that mesoderm induction establishes the lineage-specific pattern of ligand and receptor gene expression.
The Xenopus platelet-derived growth factor alpha receptor: cDNA cloning and demonstration that mesoderm induction establishes the lineage-specific pattern of ligand and receptor gene expression.
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非洲爪蟾血小板衍生生长因子 α 受体：cDNA 克隆和证明中胚层诱导建立了配体和受体基因表达的谱系特异性模式。
DOI：
10.1002/dvg.1020140305
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Mercola,M
中科院分区：
文献类型：
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作者：
Jones,SD;Ho,L;Smith,JC;Yordan,C;Stiles,CD;Mercola,M
我们克隆了Xenopus PDGF α受体cDNA，并将该克隆与编码PDGF A的cDNA一起沿着用于检测它们在Xenopus胚胎中的表达模式，并确定与谱系特异性有关的因素。COS细胞中表达的重组爪蟾α受体具有PDGF-A依赖性酪氨酸激酶活性。我们发现，受体mRNA存在于培养的边缘区组织外植体和动物帽组织诱导形成中胚层无论是通过移植到植物组织或重组激活素A处理。相比之下，PDGF A mRNA在培养的、未处理的动物帽组织中表达，并被中胚层诱导抑制。这些结果表明，外胚层产生的PDGF A可以在原肠胚形成过程中作用于中胚层，并且中胚层诱导建立了配体和受体表达的组织模式。© 1993 Wiley利斯公司
We have cloned theXenopusPDGF α receptor cDNA and have used this clone, along with cDNA encoding PDGF A, to examine their expression pattern inXenopusembryos and to determine the factors responsible for lineage specificity. Recombinant Xenopus α receptor expressed in COS cells exhibits PDGF‐A‐dependent tyrosine kinase activity. We find that receptor mRNA is present in cultured marginal zone tissue explants and in animal cap tissue induced to form mesoderm either by grafting to vegetal tissue or by treatment with recombinant activin A. In contrast, PDGF A mRNA is expressed in cultured, untreated animal cap tissue and is suppressed by mesoderm induction. These results suggest that ectodermally produced PDGF A may act on the mesoderm during gastrulation and that mesoderm induction establishes the tissue pattern of ligand and receptor expression. © 1993Wiley‐Liss, Inc.