LYMPHOCYTE STIMULATION - RAPID MULTIPARAMETER ANALYSIS
LYMPHOCYTE STIMULATION - RAPID MULTIPARAMETER ANALYSIS
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DOI：
10.1073/pnas.73.8.2881
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发表时间：
1976-01-01
影响因子：
11.1
通讯作者：
MELAMED, MR
中科院分区：
文献类型：
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作者：
DARZYNKIEWICZ, Z;TRAGANOS, F;MELAMED, MR
用异染荧光染料吖啶橙子对细胞DNA和RNA进行差异染色后，通过流式细胞荧光测定法同时测定单个淋巴细胞刺激的几个参数。该方法提供了一种分析受刺激细胞在细胞周期（G1、S和G2 + M）中进展的方法，此外还测量了每个细胞的RNA积累; RNA参数可用于区分G1和G 0细胞。多参数分析可能是独特的价值，在歧视的情况下，淋巴细胞对刺激物的转录和增殖反应不相关。
Several parameters of stimulation of individual lymphocytes [human] were measured simultaneously by flow-cytofluorometry after differential staining of cellular DNA and RNA with the metachromatic fluorescent dye acridine orange. The method provides a means of analyzing the progression of stimulated cells through the cell cycle (G1, S and G2 + M), in addition to measuring the accumulation of RNA per cell; the RNA parameter is useful in distinguishing G1 from G0 cells. The multiparameter analysis may be of unique value in discriminating cases where the transcriptional and proliferative responses of lymphocytes to stimulants are not correlated.