A phosphorylated cytoplasmic autoantigen, GW182, associates with a unique population of human mRNAs within novel cytoplasmic speckles
A phosphorylated cytoplasmic autoantigen, GW182, associates with a unique population of human mRNAs within novel cytoplasmic speckles
复制标题
DOI：
10.1091/mbc.01-11-0544
复制
发表时间：
2002-04-01
影响因子：
3.3
通讯作者：
Fritzler, MJ
中科院分区：
文献类型：
--
作者：
Eystathioy, T;Chan, EKL;Fritzler, MJ
一种新的人类细胞结构已经被确定，它包含一种独特的自身免疫抗原和多种信使rna。该复合物是在一名运动和感觉神经病变患者的自身免疫血清中发现的，含有182 kDa的蛋白质。该基因和编码该蛋白的cDNA显示一个开放的阅读框，具有甘氨酸-色氨酸（GW）重复序列和单个RNA识别基序。患者血清和家兔血清对抗重组GW蛋白时，均含有被称为GW小体（gwb）的离散细胞质斑点，这些斑点不与高尔基复合物、核内体、溶酶体或过氧化物酶体重叠。与GW182相关的mrna代表了一组转录本，这些转录本被认为存在于GW复合物中。我们提出GW核糖核蛋白复合物通过隔离参与细胞生长和稳态的特定基因转录物亚群，参与基因表达的转录后调控。
A novel human cellular structure has been identified that contains a unique autoimmune antigen and multiple messenger RNAs. This complex was discovered using an autoimmune serum from a patient with motor and sensory neuropathy and contains a protein of 182 kDa. The gene and cDNA encoding the protein indicated an open reading frame with glycine-tryptophan (GW) repeats and a single RNA recognition motif. Both the patient's serum and a rabbit serum raised against the recombinant GW protein costained discrete cytoplasmic speckles designated as GW bodies (GWBs) that do not overlap with the Golgi complex, endosomes, lysosomes, or peroxisomes. The mRNAs associated with GW182 represent a clustered set of transcripts that are presumed to reside within the GW complexes. We propose that the GW ribonucleoprotein complex is involved in the posttranscriptional regulation of gene expression by sequestering a specific subset of gene transcripts involved in cell growth and homeostasis.